Identificador persistente para citar o vincular este elemento:
http://hdl.handle.net/10553/48293
Campo DC | Valor | idioma |
---|---|---|
dc.contributor.author | Gallardo Campos, Germán | en_US |
dc.contributor.author | Tabraue, C. | en_US |
dc.contributor.author | Quintana, J | en_US |
dc.contributor.author | López Blanco, Félix | en_US |
dc.contributor.author | Cabrera, J. | en_US |
dc.contributor.author | Díaz, R. | en_US |
dc.contributor.author | Estévez, F. | en_US |
dc.contributor.author | Ruiz de Galarreta, C. M. | en_US |
dc.contributor.author | Fanjul Rodríguez, Luisa Fernanda | en_US |
dc.contributor.author | Santana, P. | en_US |
dc.contributor.other | Cabrera, Javier | - |
dc.contributor.other | Quintana, Jose | - |
dc.contributor.other | Tabraue, Carlos | - |
dc.contributor.other | Estevez, Francisco | - |
dc.date.accessioned | 2018-11-23T20:29:43Z | - |
dc.date.available | 2018-11-23T20:29:43Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.issn | 0145-5680 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/48293 | - |
dc.description.abstract | Ceramide has emerged as a pleiotropic signal mediator of cellular responses including differentiation, proliferation, cell cycle arrest and apoptosis. In the present study we evaluated the effect of cell permeant ceramide analogues on ligand-induced tyrosine phosphorylation of the EGF receptor (EGFR), phospholipase C gamma (PLC gamma) activity and cell proliferation. Treatment with N-acetylsphingosine (C2-cer) and N-hexanoylceramide (C6-cer) prevented EGF-induced tyrosine trans-phosphorylation of the receptor in two different cell lines overexpressing the human EGFR (A431 and EGF-T17 cells). In contrast, treatment of A431 and EGFR-T17 cells with C2-cer or C6-cer did not affect the ligand binding capacity of the receptor, an effect that was however: observed after TPA-induced activation of PKC. In addition EGF-stimulated PLC gamma activity was transiently decreased in A431 cells treated with C6-cer and only a modest, albeit significant reduction on ligand-induced H-3-InsP(3) generation was observed in EGFR-T17 cells pretreated with ceramide. We also examined the effect of C2-cer on serum (A431)- or EGF (EGFR-T17)-induced cell proliferation Treatment of EGFR-T17 cells with C2-cer (0.1-10 muM) did not affect cell viability, but prevented EGF-induced H-3-thymidine incorporation in a dose-dependent manner. In contrast, H-3-thymidine incorporation in serum-stimulated A431 cells decreased only at the higher doses of C2-cer used (1-10 muM), being this effect accompanied by a slight, albeit significant (20-25%), reduction in cell viability. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Cellular and Molecular Biology | en_US |
dc.source | Cellular and molecular biology (Noisy-le-Grand, France), [ISSN 0145-5680], v. 46, p. 1305-1312, (Enero 2020). | en_US |
dc.subject | 32 Ciencias médicas | en_US |
dc.subject | 2407 Biología celular | en_US |
dc.subject.other | Activated Protein-Kinase | en_US |
dc.subject.other | Tumor-Necrosis-Factor | en_US |
dc.subject.other | Egf-Receptor | en_US |
dc.subject.other | Induced Apoptosis | en_US |
dc.subject.other | Tyrosine Kinase | en_US |
dc.subject.other | Fatty-Acids | en_US |
dc.subject.other | Phosphorylation | en_US |
dc.subject.other | Sphingomyelinase | en_US |
dc.subject.other | 2Nd-Messengers | en_US |
dc.subject.other | Sphingolipids | en_US |
dc.title | Regulation by ceramide of epidermal growth factor signal transduction and mitogenesis in cell lines overexpressing the growth factor receptor | en_US |
dc.type | info:eu-repo/semantics/Article | en_US |
dc.type | Article | en_US |
dc.identifier.scopus | 0034329854 | - |
dc.identifier.isi | 000165108400017 | - |
dcterms.isPartOf | Cellular And Molecular Biology | - |
dcterms.source | Cellular And Molecular Biology[ISSN 0145-5680],v. 46 (7), p. 1305-1312 | - |
dc.contributor.authorscopusid | 6603046608 | - |
dc.contributor.authorscopusid | 6506833060 | - |
dc.contributor.authorscopusid | 8681043500 | - |
dc.contributor.authorscopusid | 6602478628 | - |
dc.contributor.authorscopusid | 35598975600 | - |
dc.contributor.authorscopusid | 7201925856 | - |
dc.contributor.authorscopusid | 7201925856 | - |
dc.contributor.authorscopusid | 7003810011 | - |
dc.contributor.authorscopusid | 7003806034 | - |
dc.contributor.authorscopusid | 7004158812 | - |
dc.contributor.authorscopusid | 7003526778 | - |
dc.description.lastpage | 1312 | en_US |
dc.description.firstpage | 1305 | en_US |
dc.relation.volume | 46 | en_US |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.identifier.wos | WOS:000165108400017 | - |
dc.contributor.daisngid | 7801524 | - |
dc.contributor.daisngid | 2294555 | - |
dc.contributor.daisngid | 4816905 | - |
dc.contributor.daisngid | 128315 | - |
dc.contributor.daisngid | 8088814 | - |
dc.contributor.daisngid | 2864371 | - |
dc.contributor.daisngid | 240124 | - |
dc.contributor.daisngid | 13600456 | - |
dc.contributor.daisngid | 384944 | - |
dc.contributor.daisngid | 1664323 | - |
dc.contributor.daisngid | 1127140 | - |
dc.contributor.daisngid | 329218 | - |
dc.contributor.daisngid | 31449715 | - |
dc.identifier.investigatorRID | L-9179-2014 | - |
dc.identifier.investigatorRID | K-5709-2014 | - |
dc.identifier.investigatorRID | D-7126-2015 | - |
dc.identifier.investigatorRID | K-5125-2014 | - |
dc.description.numberofpages | 8 | en_US |
dc.utils.revision | Sí | en_US |
dc.contributor.wosstandard | WOS:Gallardo, G | - |
dc.contributor.wosstandard | WOS:Tabraue, C | - |
dc.contributor.wosstandard | WOS:Quintana, J | - |
dc.contributor.wosstandard | WOS:Lopez-Blanco, F | - |
dc.contributor.wosstandard | WOS:Cabrera, J | - |
dc.contributor.wosstandard | WOS:Diaz, R | - |
dc.contributor.wosstandard | WOS:Estevez, F | - |
dc.contributor.wosstandard | WOS:de Galarreta, CMR | - |
dc.contributor.wosstandard | WOS:Fanjul, LF | - |
dc.contributor.wosstandard | WOS:Santana, P | - |
dc.date.coverdate | Enero 2000 | en_US |
dc.identifier.ulpgc | Sí | en_US |
dc.contributor.buulpgc | BU-MED | en_US |
dc.description.jcr | 1,449 | - |
dc.description.jcrq | Q3 | - |
dc.description.scie | SCIE | - |
item.fulltext | Sin texto completo | - |
item.grantfulltext | none | - |
crisitem.author.dept | GIR IATEXT: Didáctica, Aprendizaje y Motivación en Contextos Específicos | - |
crisitem.author.dept | IU de Análisis y Aplicaciones Textuales | - |
crisitem.author.dept | Departamento de Didácticas Específicas | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Morfología | - |
crisitem.author.dept | GIR IUIBS: Bioquímica | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Ciencias Clínicas | - |
crisitem.author.dept | GIR IUIBS: Bioquímica | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.dept | GIR IUIBS: Bioquímica | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | Departamento de Bioquímica y Biología Molecular, Fisiología, Genética e Inmunología | - |
crisitem.author.orcid | 0000-0002-1516-1750 | - |
crisitem.author.orcid | 0000-0001-9920-8116 | - |
crisitem.author.orcid | 0000-0001-8225-4538 | - |
crisitem.author.orcid | 0000-0003-1167-9787 | - |
crisitem.author.orcid | 0000-0002-9728-2774 | - |
crisitem.author.orcid | 0009-0000-1982-055X | - |
crisitem.author.orcid | 0000-0002-4093-2692 | - |
crisitem.author.parentorg | IU de Análisis y Aplicaciones Textuales | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.fullName | Gallardo Campos, Germán | - |
crisitem.author.fullName | Tabraue Tarbay, Carlos | - |
crisitem.author.fullName | Quintana Aguiar, José Martín | - |
crisitem.author.fullName | López Blanco, Félix | - |
crisitem.author.fullName | Estévez Rosas, Francisco Jesús | - |
crisitem.author.fullName | Fanjul Rodríguez, Luisa Fernanda | - |
crisitem.author.fullName | Santana Delgado, María Del Pino | - |
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