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Title: Cutaneous lesions in experimental acute and subacute African swine fever: An immunohistopathological and ultrastructural study
Authors: Mozos Mora, Elena
Herráez Thomas, Pedro Manuel 
Pérez Arevalo,Jose 
Fernández Rodríguez, Antonio Jesús 
Blanco Rodríguez, Alfonso
Martín, M. P.
Jover Moyano, Amador
UNESCO Clasification: 240111 Patología animal
310907 Patología
Keywords: Experimental African swine fever
Issue Date: 2003
Publisher: 0341-6593
Journal: Deutsche tierarztliche Wochenschrift 
Abstract: Histopathological, immunohistochemical and ultrastructural cutaneous changes are described in acute and subacute forms of experimental African Swine Fever (ASF). Fourteen 3-month-old Large White cross pigs were infected intramuscularly with the Dominican Republic 78 (DR78) ASF virus isolate and euthanised in pairs on alternatives days (3 to 17) post-inoculation (dpi). Three pigs were found dead at 8, 10 and 13 dpi, respectively. Antibodies against viral antigen Vp73, human fibrinogen, glycoprotein IIIa and Factor VIII-ra were used to evaluate viral antigen distribution, fibrin microthrombi and platelets in dermal vessels, respectively. Cutaneous lesions were characterised by vascular changes ranging from hyperaemia, mild oedema, scarce fibrin microthrombi and microhaemorrhages in euthanised animals, to generalized fibrin microthrombosis and microhaemorrhages in dead pigs. Secondary pustules and superficial folliculitis were observed in two animals dead at 10 and 13 dpi. Diffuse cytoplasmic Vp73 labelling was found in numerous intravascular monocytes and dermal macrophages. Ultrastructural studies showed mature viral particles in the lumen of dermal vessels but viral replication was not observed; nonetheless, microtubuloreticular structures were observed in the cytoplasm of some endothelial cells and macrophages which showed cytopathic effects, signs of cell activation or degeneration. Morphological and immunohistochemical evidences of platelet activation, degranulation and consumption were observed from 5 dpi onward.
ISSN: 0341-6593
Source: Deutsche Tierarztliche Wochenschrift [ISSN 0341-6593], v. 110 (4), p. 150-154
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