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Title: Cross-reactivity of human and bovine antibodies in striped dolphin paraffin wax-embedded tissues
Authors: Jáber Mohamad, José Raduán 
Fernández Rodríguez, Antonio Jesús 
Herráez Thomas, Pedro Manuel 
Espinosa De Los Monteros Y Zayas, Antonio 
Ramírez, Gustavo Adolfo
García, Patricia M.
Fernández, T. 
Arbelo Hernández, Manuel Antonio 
Pérez Arevalo,Jose 
UNESCO Clasification: 310903 Inmunología
2412 Inmunología
241202 Anticuerpos
240118 Mamíferos
Keywords: Dolphin
Parasite cholangitis
Non-specific chronic reactive hepatitis
Issue Date: 2003
Publisher: 0165-2427
Journal: Veterinary Immunology and Immunopathology 
Abstract: This study evaluates the cross-reactivity of seven anti-human and one anti-bovine antibodies in formalin-fixed, paraffin-embedded tissue samples of liver and mesenteric lymph nodes of 13 striped dolphins (Stenella coeruleoalba). Four antibodies (CD3, IgG, lysozyme and S100 protein) reacted with striped dolphin lymph nodes in a similar pattern to that observed in thespecies of origin. The anti-human MHC class II mAb reacted strongly with macrophages and dendritic-like cells of stripeddolphins, whereas a small number of lymphocytes were labelled with this antibody. These antibodies were used to study theimmunophenotype of the inflammatory infiltrated in non-specific chronic reactive hepatitis (eight cases) and chronic parasitecholangitis (two cases) and normal liver (three cases) of striped dolphins. Non-specific chronic reactive hepatitis was composed ofinflammatory infiltration of CD3+ T lymphocytes and IgG+ plasma cells in portal spaces and hepatic sinusoids. Lymphonodular aggregates observed in chronic parasitic cholangitis showed a cellular distribution similar to that found in lymph node cortex, including the presence of S100+ and MHC class II+ dendritic-like cells in lymphoid follicles and interfollicular areas. This result suggests that those inflammatory infiltrates are highly organised to enhance antigen presentation to B and T cells.
ISSN: 0165-2427
DOI: 10.1016/S0165-2427(03)00158-2
Source: Veterinary Immunology and Immunopathology [ISSN 0165-2427], v. 96 (1-2), p. 65-72
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