Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47547
Title: Cross-reactivity of human and bovine antibodies in striped dolphin paraffin wax-embedded tissues
Authors: Jáber Mohamad, José Raduán 
Fernández Rodríguez, Antonio Jesús 
Herráez Thomas, Pedro Manuel 
Espinosa De Los Monteros Y Zayas, Antonio 
Ramírez, Gustavo Adolfo
García, Patricia M.
Fernández, T. 
Arbelo Hernández, Manuel Antonio 
Pérez Arevalo,Jose 
UNESCO Clasification: 310903 Inmunología
2412 Inmunología
241202 Anticuerpos
240118 Mamíferos
Keywords: Dolphin
Cross-reactivity
Leukocytes
Parasite cholangitis
Non-specific chronic reactive hepatitis
Issue Date: 2003
Publisher: 0165-2427
Journal: Veterinary Immunology and Immunopathology 
Abstract: This study evaluates the cross-reactivity of seven anti-human and one anti-bovine antibodies in formalin-fixed, paraffin-embedded tissue samples of liver and mesenteric lymph nodes of 13 striped dolphins (Stenella coeruleoalba). Four antibodies (CD3, IgG, lysozyme and S100 protein) reacted with striped dolphin lymph nodes in a similar pattern to that observed in thespecies of origin. The anti-human MHC class II mAb reacted strongly with macrophages and dendritic-like cells of stripeddolphins, whereas a small number of lymphocytes were labelled with this antibody. These antibodies were used to study theimmunophenotype of the inflammatory infiltrated in non-specific chronic reactive hepatitis (eight cases) and chronic parasitecholangitis (two cases) and normal liver (three cases) of striped dolphins. Non-specific chronic reactive hepatitis was composed ofinflammatory infiltration of CD3+ T lymphocytes and IgG+ plasma cells in portal spaces and hepatic sinusoids. Lymphonodular aggregates observed in chronic parasitic cholangitis showed a cellular distribution similar to that found in lymph node cortex, including the presence of S100+ and MHC class II+ dendritic-like cells in lymphoid follicles and interfollicular areas. This result suggests that those inflammatory infiltrates are highly organised to enhance antigen presentation to B and T cells.
URI: http://hdl.handle.net/10553/47547
ISSN: 0165-2427
DOI: 10.1016/S0165-2427(03)00158-2
Source: Veterinary Immunology and Immunopathology [ISSN 0165-2427], v. 96 (1-2), p. 65-72
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

25
checked on Mar 24, 2024

WEB OF SCIENCETM
Citations

22
checked on Jul 9, 2023

Page view(s)

62
checked on Jan 13, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.