Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47245
Title: Invasion and survival of Photobacterium damselae subsp. piscicida in non-phagocytic cells of gilthead sea bream, sparus aurata L
Authors: Acosta, F. 
Vivas, J.
Padilla, D. 
Vega, J.
Bravo, Jimena 
Grasso, V.
Real Valcárcel, Fernando 
Keywords: Pathogen Pasteurella-Piscicida
Bass Macrophages
Fish
Seabream
Neutrophils, et al
Issue Date: 2009
Publisher: 0140-7775
Journal: Journal of Fish Diseases 
Abstract: Fluorescence microscopy and gentamicin protection assays were used to investigate the ability of four Photobacterium damselae subsp. pisicida (Phdp) strains to adhere to and to invade the fish epithelial cell line, SAF-1, derived from Sparus aurata. All strains tested were detected intracellularly using both techniques, although internalization levels varied among strains. Treatment with cytochalasin D and experiments carried out at 4 degrees C demonstrated that a functional host cell cytoskeleton and active cell metabolism are necessary for bacterial internalization. Intracellular bacteria were detected for up to 7 days with a round morphology and were stained with DAPI, indicating that some bacterial cells may remain viable inside SAF-1 cells. Our in vitro findings indicate that Phdp are capable of adhering, entering and surviving within the non-phagocytic epithelial cell line SAF-1, which may be important for persistence and establishment of a carrier state in S. aurata.
URI: http://hdl.handle.net/10553/47245
ISSN: 0140-7775
DOI: 10.1111/j.1365-2761.2009.01023.x
Source: Journal of Fish Diseases[ISSN 0140-7775],v. 32, p. 535-541
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