Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/47245
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Acosta, F. | en_US |
dc.contributor.author | Vivas, J. | en_US |
dc.contributor.author | Padilla, D. | en_US |
dc.contributor.author | Vega, J. | en_US |
dc.contributor.author | Bravo, Jimena | en_US |
dc.contributor.author | Grasso, V. | en_US |
dc.contributor.author | Real Valcárcel, Fernando | en_US |
dc.date.accessioned | 2018-11-23T11:59:51Z | - |
dc.date.available | 2018-11-23T11:59:51Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.issn | 0140-7775 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/47245 | - |
dc.description.abstract | Fluorescence microscopy and gentamicin protection assays were used to investigate the ability of four Photobacterium damselae subsp. pisicida (Phdp) strains to adhere to and to invade the fish epithelial cell line, SAF-1, derived from Sparus aurata. All strains tested were detected intracellularly using both techniques, although internalization levels varied among strains. Treatment with cytochalasin D and experiments carried out at 4 degrees C demonstrated that a functional host cell cytoskeleton and active cell metabolism are necessary for bacterial internalization. Intracellular bacteria were detected for up to 7 days with a round morphology and were stained with DAPI, indicating that some bacterial cells may remain viable inside SAF-1 cells. Our in vitro findings indicate that Phdp are capable of adhering, entering and surviving within the non-phagocytic epithelial cell line SAF-1, which may be important for persistence and establishment of a carrier state in S. aurata. | en_US |
dc.language | eng | en_US |
dc.publisher | 0140-7775 | |
dc.relation.ispartof | Journal of Fish Diseases | en_US |
dc.source | Journal of Fish Diseases[ISSN 0140-7775],v. 32, p. 535-541 | en_US |
dc.subject.other | Pathogen Pasteurella-Piscicida | en_US |
dc.subject.other | Bass Macrophages | en_US |
dc.subject.other | Fish | en_US |
dc.subject.other | Seabream | en_US |
dc.subject.other | Neutrophils | en_US |
dc.subject.other | Virulence | en_US |
dc.subject.other | Capsule | en_US |
dc.subject.other | Serum | en_US |
dc.title | Invasion and survival of Photobacterium damselae subsp. piscicida in non-phagocytic cells of gilthead sea bream, sparus aurata L | en_US |
dc.type | info:eu-repo/semantics/Article | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1111/j.1365-2761.2009.01023.x | en_US |
dc.identifier.scopus | 66149084464 | - |
dc.identifier.isi | 000266243200006 | - |
dc.contributor.authorscopusid | 56269311600 | - |
dc.contributor.authorscopusid | 7006473722 | - |
dc.contributor.authorscopusid | 7005088705 | - |
dc.contributor.authorscopusid | 35068773000 | - |
dc.contributor.authorscopusid | 23059544100 | - |
dc.contributor.authorscopusid | 23060092500 | - |
dc.contributor.authorscopusid | 7005397318 | - |
dc.description.lastpage | 541 | en_US |
dc.description.firstpage | 535 | en_US |
dc.relation.volume | 32 | en_US |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.contributor.daisngid | 878226 | - |
dc.contributor.daisngid | 1854108 | - |
dc.contributor.daisngid | 31455203 | - |
dc.contributor.daisngid | 7394693 | - |
dc.contributor.daisngid | 1844262 | - |
dc.contributor.daisngid | 345205 | - |
dc.contributor.daisngid | 29464210 | - |
dc.utils.revision | Sí | en_US |
dc.contributor.wosstandard | WOS:Acosta, F | - |
dc.contributor.wosstandard | WOS:Vivas, J | - |
dc.contributor.wosstandard | WOS:Padilla, D | - |
dc.contributor.wosstandard | WOS:Vega, J | - |
dc.contributor.wosstandard | WOS:Bravo, J | - |
dc.contributor.wosstandard | WOS:Grasso, V | - |
dc.contributor.wosstandard | WOS:Real, F | - |
dc.date.coverdate | Junio 2009 | en_US |
dc.identifier.ulpgc | Sí | en_US |
dc.contributor.buulpgc | BU-VET | en_US |
dc.description.jcr | 1,697 | |
dc.description.jcrq | Q1 | |
dc.description.scie | SCIE | |
item.grantfulltext | none | - |
item.fulltext | Sin texto completo | - |
crisitem.author.dept | GIR ECOAQUA: Grupo de Investigación en Acuicultura | - |
crisitem.author.dept | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.dept | GIR IUSA-ONE HEALTH 2.-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria | - |
crisitem.author.dept | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.dept | GIR IUSA-ONE HEALTH 2.-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria | - |
crisitem.author.dept | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.dept | Departamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos | - |
crisitem.author.orcid | 0000-0002-1098-7529 | - |
crisitem.author.orcid | 0000-0002-6678-5029 | - |
crisitem.author.orcid | 0000-0002-3237-1020 | - |
crisitem.author.orcid | 0000-0001-6526-0354 | - |
crisitem.author.parentorg | IU de Investigación en Acuicultura Sostenible y Ec | - |
crisitem.author.parentorg | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.parentorg | IU de Sanidad Animal y Seguridad Alimentaria | - |
crisitem.author.fullName | Acosta Arbelo, Félix Antonio | - |
crisitem.author.fullName | Padilla Castillo, Daniel Fermín | - |
crisitem.author.fullName | Bravo García, Jimena | - |
crisitem.author.fullName | Real Valcárcel, Fernando | - |
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