Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/47245
DC FieldValueLanguage
dc.contributor.authorAcosta, F.en_US
dc.contributor.authorVivas, J.en_US
dc.contributor.authorPadilla, D.en_US
dc.contributor.authorVega, J.en_US
dc.contributor.authorBravo, Jimenaen_US
dc.contributor.authorGrasso, V.en_US
dc.contributor.authorReal Valcárcel, Fernandoen_US
dc.date.accessioned2018-11-23T11:59:51Z-
dc.date.available2018-11-23T11:59:51Z-
dc.date.issued2009en_US
dc.identifier.issn0140-7775en_US
dc.identifier.urihttp://hdl.handle.net/10553/47245-
dc.description.abstractFluorescence microscopy and gentamicin protection assays were used to investigate the ability of four Photobacterium damselae subsp. pisicida (Phdp) strains to adhere to and to invade the fish epithelial cell line, SAF-1, derived from Sparus aurata. All strains tested were detected intracellularly using both techniques, although internalization levels varied among strains. Treatment with cytochalasin D and experiments carried out at 4 degrees C demonstrated that a functional host cell cytoskeleton and active cell metabolism are necessary for bacterial internalization. Intracellular bacteria were detected for up to 7 days with a round morphology and were stained with DAPI, indicating that some bacterial cells may remain viable inside SAF-1 cells. Our in vitro findings indicate that Phdp are capable of adhering, entering and surviving within the non-phagocytic epithelial cell line SAF-1, which may be important for persistence and establishment of a carrier state in S. aurata.en_US
dc.languageengen_US
dc.publisher0140-7775
dc.relation.ispartofJournal of Fish Diseasesen_US
dc.sourceJournal of Fish Diseases[ISSN 0140-7775],v. 32, p. 535-541en_US
dc.subject.otherPathogen Pasteurella-Piscicidaen_US
dc.subject.otherBass Macrophagesen_US
dc.subject.otherFishen_US
dc.subject.otherSeabreamen_US
dc.subject.otherNeutrophilsen_US
dc.subject.otherVirulenceen_US
dc.subject.otherCapsuleen_US
dc.subject.otherSerumen_US
dc.titleInvasion and survival of Photobacterium damselae subsp. piscicida in non-phagocytic cells of gilthead sea bream, sparus aurata Len_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/j.1365-2761.2009.01023.xen_US
dc.identifier.scopus66149084464-
dc.identifier.isi000266243200006-
dc.contributor.authorscopusid56269311600-
dc.contributor.authorscopusid7006473722-
dc.contributor.authorscopusid7005088705-
dc.contributor.authorscopusid35068773000-
dc.contributor.authorscopusid23059544100-
dc.contributor.authorscopusid23060092500-
dc.contributor.authorscopusid7005397318-
dc.description.lastpage541en_US
dc.description.firstpage535en_US
dc.relation.volume32en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid878226-
dc.contributor.daisngid1854108-
dc.contributor.daisngid31455203-
dc.contributor.daisngid7394693-
dc.contributor.daisngid1844262-
dc.contributor.daisngid345205-
dc.contributor.daisngid29464210-
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Acosta, F-
dc.contributor.wosstandardWOS:Vivas, J-
dc.contributor.wosstandardWOS:Padilla, D-
dc.contributor.wosstandardWOS:Vega, J-
dc.contributor.wosstandardWOS:Bravo, J-
dc.contributor.wosstandardWOS:Grasso, V-
dc.contributor.wosstandardWOS:Real, F-
dc.date.coverdateJunio 2009en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.jcr1,697
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR ECOAQUA: Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONE HEALTH 2.-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR IUSA-ONE HEALTH 2.-Sanidad Animal de la Acuicultura y Especies Silvestres, Enfermedades Infecciosas y Seguridad Alimentaria-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-1098-7529-
crisitem.author.orcid0000-0002-6678-5029-
crisitem.author.orcid0000-0002-3237-1020-
crisitem.author.orcid0000-0001-6526-0354-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameAcosta Arbelo, Félix Antonio-
crisitem.author.fullNamePadilla Castillo, Daniel Fermín-
crisitem.author.fullNameBravo García, Jimena-
crisitem.author.fullNameReal Valcárcel, Fernando-
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