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Title: New approach for the clinical monitoring of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972
Authors: Plíšek, Jiří
Krčmová, Lenka Kujovská
Aufartová, Jana
Morales, Tanausú V.
Esponda, Sarah M. 
Oros, Roman
Kasalová, Eva
Santana-Rodriguez, Jose J. 
Sobotka, Luboš
Solich, Petr
Solichová, Dagmar
UNESCO Clasification: 2301 química analítica
Keywords: Clinical research
Mass spectrometry
Standard reference material 972
Vitamin D
Issue Date: 2013
Publisher: 1615-9306
Journal: Journal of Separation Science 
Abstract: Biomarkers, 25‐hydroxyvitamin D3 and 25‐hydroxyvitamin D2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra‐HPLC with MS/MS methodology for the analysis of 25‐hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25‐hydroxyvitamin D3 and 25‐hydroxyvitamin D2, respectively. Intra‐assay precision (%RSD) was lower than 4.5%, and inter‐assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non‐agenarian patients, patients with familial hypercholesterolemia and patients suffering from age‐related macular degeneration.
ISSN: 1615-9306
DOI: 10.1002/jssc.201300553
Source: Journal of Separation Science [ISSN 1615-9306], v. 36, p. 3702-3708
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