Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/47126
Campo DC Valoridioma
dc.contributor.authorPlíšek, Jiříen_US
dc.contributor.authorKrčmová, Lenka Kujovskáen_US
dc.contributor.authorAufartová, Janaen_US
dc.contributor.authorMorales, Tanausú V.en_US
dc.contributor.authorEsponda, Sarah M.en_US
dc.contributor.authorOros, Romanen_US
dc.contributor.authorKasalová, Evaen_US
dc.contributor.authorSantana-Rodriguez, Jose J.en_US
dc.contributor.authorSobotka, Lubošen_US
dc.contributor.authorSolich, Petren_US
dc.contributor.authorSolichová, Dagmaren_US
dc.date.accessioned2018-11-23T11:03:14Z-
dc.date.available2018-11-23T11:03:14Z-
dc.date.issued2013en_US
dc.identifier.issn1615-9306en_US
dc.identifier.urihttp://hdl.handle.net/10553/47126-
dc.description.abstractBiomarkers, 25‐hydroxyvitamin D3 and 25‐hydroxyvitamin D2, are important indicators of the vitamin D general status and are monitored in several pathophysiological disorders, such as osteoporosis, diabetes, heart disease, etc. A novel ultra‐HPLC with MS/MS methodology for the analysis of 25‐hydroxyvitamin D derivatives coupled with a very simple and highly rapid sample preparation step was developed. Analytical parameters obtained showed linearity (R2) above 0.999 for both vitamins with accuracies between 95.8 and 102%. The LODs were as low as 0.22 and 0.67 nmol/L for 25‐hydroxyvitamin D3 and 25‐hydroxyvitamin D2, respectively. Intra‐assay precision (%RSD) was lower than 4.5%, and inter‐assay precision (%RSD) was lower than 6.5%. The feasibility of the developed methodology to be applied in clinical routine analysis has been proved by its application in blood samples from non‐agenarian patients, patients with familial hypercholesterolemia and patients suffering from age‐related macular degeneration.en_US
dc.languageengen_US
dc.publisher1615-9306-
dc.relation.ispartofJournal of Separation Scienceen_US
dc.sourceJournal of Separation Science [ISSN 1615-9306], v. 36, p. 3702-3708en_US
dc.subject2301 química analíticaen_US
dc.subject.otherClinical researchen_US
dc.subject.otherMass spectrometryen_US
dc.subject.otherStandard reference material 972en_US
dc.subject.otherVitamin Den_US
dc.titleNew approach for the clinical monitoring of 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 by ultra high performance liquid chromatography with MS/MS based on the standard reference material 972en_US
dc.typeinfo:eu-repo/semantics/Articlees
dc.typeArticlees
dc.identifier.doi10.1002/jssc.201300553
dc.identifier.scopus84889643059-
dc.identifier.isi000327556300008
dc.contributor.authorscopusid36127932200-
dc.contributor.authorscopusid15071897900-
dc.contributor.authorscopusid36187944300-
dc.contributor.authorscopusid36454811400-
dc.contributor.authorscopusid57201412739-
dc.contributor.authorscopusid55955016100-
dc.contributor.authorscopusid55954992500-
dc.contributor.authorscopusid56248783900-
dc.contributor.authorscopusid7005048316-
dc.contributor.authorscopusid7003997787-
dc.contributor.authorscopusid6701818464-
dc.description.lastpage3708-
dc.description.firstpage3702-
dc.relation.volume36-
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.contributor.daisngid2102416
dc.contributor.daisngid524355
dc.contributor.daisngid3089112
dc.contributor.daisngid30794982
dc.contributor.daisngid23299015
dc.contributor.daisngid30773546
dc.contributor.daisngid6396191
dc.contributor.daisngid10914640
dc.contributor.daisngid371672
dc.contributor.daisngid88909
dc.contributor.daisngid193151
dc.contributor.wosstandardWOS:Plisek, J
dc.contributor.wosstandardWOS:Krcmova, LK
dc.contributor.wosstandardWOS:Aufartova, J
dc.contributor.wosstandardWOS:Morales, TV
dc.contributor.wosstandardWOS:Esponda, SM
dc.contributor.wosstandardWOS:Oros, R
dc.contributor.wosstandardWOS:Kasalova, E
dc.contributor.wosstandardWOS:Santana-Rodriguez, JJ
dc.contributor.wosstandardWOS:Sobotka, L
dc.contributor.wosstandardWOS:Solich, P
dc.contributor.wosstandardWOS:Solichova, D
dc.date.coverdateDiciembre 2013
dc.identifier.ulpgces
dc.description.sjr1,119
dc.description.jcr2,594
dc.description.sjrqQ2
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IUNAT: Análisis Químico Medioambiental-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptDepartamento de Química-
crisitem.author.deptGIR IUNAT: Análisis Químico Medioambiental-
crisitem.author.deptIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.deptDepartamento de Química-
crisitem.author.orcid0000-0001-9872-5293-
crisitem.author.orcid0000-0002-5635-7215-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.parentorgIU de Estudios Ambientales y Recursos Naturales-
crisitem.author.fullNameMontesdeoca Esponda, Sarah-
crisitem.author.fullNameSantana Rodríguez, José Juan-
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