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Title: Protoplast isolation from ulva rigida (Chlorophyta)
Authors: Björk, Mats
Pedersón, Marianne 
GÓmez-Pinchetti, Juan Luis 
Garcia-Blairsy Reina, Guillermo 
UNESCO Clasification: 241705 Biología marina
241719 Fisiología vegetal
241707 Algología (ficología)
Keywords: Ulva rigida
Issue Date: 1992
Publisher: 0007-1617
Journal: British phycological journal 
Abstract: High numbers of protoplasts were isolated from wild and cultivated thalli of Ulva rigida. Optimal conditions for protoplast release were obtained with 1·5% Abalone Acetone Powder and 1·5% Cellulysin in 0·4 M mannitol. Treatment for 30 min with hypotonic (0·8 M) solution more than doubled the yield. Growth conditions prior to enzyme treatment also influenced the yield greatly. Protoplasts were photosynthetically active, and 70–90% of the protoplasts were estimated to be viable.
ISSN: 0007-1617
DOI: 10.1080/00071619200650331
Source: British Phycological Journal [ISSN 0007-1617], v. 27, p. 401-407
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