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http://hdl.handle.net/10553/46901
| Título: | Protoplast isolation from ulva rigida (Chlorophyta) | Autores/as: | Björk, Mats Pedersón, Marianne GÓmez-Pinchetti, Juan Luis Garcia-Blairsy Reina, Guillermo |
Clasificación UNESCO: | 241705 Biología marina 241719 Fisiología vegetal 241707 Algología (ficología) |
Palabras clave: | Ulva rigida | Fecha de publicación: | 1992 | Editor/a: | 0007-1617 | Publicación seriada: | British phycological journal | Resumen: | High numbers of protoplasts were isolated from wild and cultivated thalli of Ulva rigida. Optimal conditions for protoplast release were obtained with 1·5% Abalone Acetone Powder and 1·5% Cellulysin in 0·4 M mannitol. Treatment for 30 min with hypotonic (0·8 M) solution more than doubled the yield. Growth conditions prior to enzyme treatment also influenced the yield greatly. Protoplasts were photosynthetically active, and 70–90% of the protoplasts were estimated to be viable. | URI: | http://hdl.handle.net/10553/46901 | ISSN: | 0007-1617 | DOI: | 10.1080/00071619200650331 | Fuente: | British Phycological Journal [ISSN 0007-1617], v. 27, p. 401-407 |
| Colección: | Artículos |
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