Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/46094
Title: Repeated muscle biopsies through a single skin incision do not elicit muscle signaling, but IL-6 mRNA and STAT3 phosphorylation increase in injured muscle
Authors: Guerra, Borja 
Carmen Gómez-Cabrera, M.
Ponce-González, Jesús Gustavo
Martinez-Bello, Vladimir E.
Guadalupe-Grau, Amelia
Santana, Alfredo 
Sebastia, Vicente
Viña, Jose
Calbet, Jose A. L. 
UNESCO Clasification: 32 Ciencias médicas
3206 Ciencias de la nutrición
Keywords: Human Skeletal-Muscle
Rheumatoid-Arthritis
In-Vitro
Activation
Interleukin-6, et al
Issue Date: 2011
Project: Integracion de Los Grupos de la Obesidad y El Síndrome Metabólico .... 
Journal: Journal of Applied Physiology 
Abstract: Repeated muscle biopsies through a single skin incision do not elicit muscle signaling, but IL-6 mRNA and STAT3 phosphorylation increase in injured muscle. J Appl Physiol 110: 1708-1715, 2011. First published March 24, 2011; doi:10.1152/japplphysiol.00091.2011.-To determine if muscle biopsies can be repeated using a single small (5-6 mm) skin incision without inducing immediate MAPK activation or inflammation in the noninjured areas, the phosphorylation of ERK1/2, p38-MAPK, c-Jun NH2-terminal kinases (JNKs), I kappa B alpha, IKK alpha, and signal transducer and activator of transcription 3 (STAT3) was examined concurrent with IL-6 mRNA in six muscle biopsies obtained from the vastus lateralis of five men. Four biopsies were obtained through the same incision (5-6 mm) from the right leg (taken at 0, 30, 123, and 126 min) and another two each from new incisions performed in the left leg (at 31 and 120 min), while the subjects rested supine. The first three biopsies from the right leg were taken similar to 3 cm apart from prebiopsied areas. The last biopsy was obtained from the same point from which the second biopsy was sampled. The three biopsies performed through the same skin incision from noninjured muscle areas showed similar levels of ERK1/2, p38-MAPK, JNK, IKK alpha, I kappa B alpha, and STAT3 phosphorylation and similar IL-6 mRNA content. There were no significant differences in the levels of ERK1/2, p38-MAPK, JNK, IKK alpha, and I kappa B alpha phosphorylation between the mean of the three biopsies obtained from the same incision and the sixth biopsy obtained from an injured area. STAT3 phosphorylation was increased by similar to 3.5-fold in the sixth biopsy compared with the mean the three biopsies obtained from the same incision (P < 0.05), and IL-6 mRNA content was increased by 1.8-fold (P < 0.05). In summary, repeated muscle biopsies can be performed through a single 5- to 6-mm skin incision without eliciting muscle signaling through cascades responding to cellular stress, inflammation, or muscle damage. STAT3 phosphorylation is an early event in the healing response to muscle injury, probably mediated by the autocrine production of IL-6.
URI: http://hdl.handle.net/10553/46094
ISSN: 8750-7587
DOI: 10.1152/japplphysiol.00091.2011
Source: Journal Of Applied Physiology [ISSN 8750-7587], v. 110 (6), p. 1708-1715
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