Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/46094
DC FieldValueLanguage
dc.contributor.authorGuerra, Borjaen_US
dc.contributor.authorCarmen Gómez-Cabrera, M.en_US
dc.contributor.authorPonce-González, Jesús Gustavoen_US
dc.contributor.authorMartinez-Bello, Vladimir E.en_US
dc.contributor.authorGuadalupe-Grau, Ameliaen_US
dc.contributor.authorSantana, Alfredoen_US
dc.contributor.authorSebastia, Vicenteen_US
dc.contributor.authorViña, Joseen_US
dc.contributor.authorCalbet, Jose A. L.en_US
dc.contributor.otherGuadalupe-Grau, Amelia-
dc.contributor.otherPonce Gonzalez, Jesus Gustavo-
dc.contributor.otherCalbet, Jose A-
dc.contributor.otherGomez-Cabrera, Maria Carmen-
dc.contributor.otherGuerra, Borja-
dc.date.accessioned2018-11-23T01:19:11Z-
dc.date.available2018-11-23T01:19:11Z-
dc.date.issued2011en_US
dc.identifier.issn8750-7587en_US
dc.identifier.urihttp://hdl.handle.net/10553/46094-
dc.description.abstractRepeated muscle biopsies through a single skin incision do not elicit muscle signaling, but IL-6 mRNA and STAT3 phosphorylation increase in injured muscle. J Appl Physiol 110: 1708-1715, 2011. First published March 24, 2011; doi:10.1152/japplphysiol.00091.2011.-To determine if muscle biopsies can be repeated using a single small (5-6 mm) skin incision without inducing immediate MAPK activation or inflammation in the noninjured areas, the phosphorylation of ERK1/2, p38-MAPK, c-Jun NH2-terminal kinases (JNKs), I kappa B alpha, IKK alpha, and signal transducer and activator of transcription 3 (STAT3) was examined concurrent with IL-6 mRNA in six muscle biopsies obtained from the vastus lateralis of five men. Four biopsies were obtained through the same incision (5-6 mm) from the right leg (taken at 0, 30, 123, and 126 min) and another two each from new incisions performed in the left leg (at 31 and 120 min), while the subjects rested supine. The first three biopsies from the right leg were taken similar to 3 cm apart from prebiopsied areas. The last biopsy was obtained from the same point from which the second biopsy was sampled. The three biopsies performed through the same skin incision from noninjured muscle areas showed similar levels of ERK1/2, p38-MAPK, JNK, IKK alpha, I kappa B alpha, and STAT3 phosphorylation and similar IL-6 mRNA content. There were no significant differences in the levels of ERK1/2, p38-MAPK, JNK, IKK alpha, and I kappa B alpha phosphorylation between the mean of the three biopsies obtained from the same incision and the sixth biopsy obtained from an injured area. STAT3 phosphorylation was increased by similar to 3.5-fold in the sixth biopsy compared with the mean the three biopsies obtained from the same incision (P < 0.05), and IL-6 mRNA content was increased by 1.8-fold (P < 0.05). In summary, repeated muscle biopsies can be performed through a single 5- to 6-mm skin incision without eliciting muscle signaling through cascades responding to cellular stress, inflammation, or muscle damage. STAT3 phosphorylation is an early event in the healing response to muscle injury, probably mediated by the autocrine production of IL-6.en_US
dc.languageengen_US
dc.relationIntegracion de Los Grupos de la Obesidad y El Síndrome Metabólico ....en_US
dc.relation.ispartofJournal of Applied Physiologyen_US
dc.sourceJournal Of Applied Physiology [ISSN 8750-7587], v. 110 (6), p. 1708-1715en_US
dc.subject32 Ciencias médicasen_US
dc.subject3206 Ciencias de la nutriciónen_US
dc.subject.otherHuman Skeletal-Muscleen_US
dc.subject.otherRheumatoid-Arthritisen_US
dc.subject.otherIn-Vitroen_US
dc.subject.otherActivationen_US
dc.subject.otherInterleukin-6en_US
dc.subject.otherExerciseen_US
dc.subject.otherCellsen_US
dc.subject.otherInflammationen_US
dc.subject.otherTranscriptionen_US
dc.subject.otherTransductionen_US
dc.titleRepeated muscle biopsies through a single skin incision do not elicit muscle signaling, but IL-6 mRNA and STAT3 phosphorylation increase in injured muscleen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1152/japplphysiol.00091.2011en_US
dc.identifier.scopus79959353579-
dc.identifier.isi000291532600028-
dcterms.isPartOfJournal Of Applied Physiology-
dcterms.sourceJournal Of Applied Physiology[ISSN 8750-7587],v. 110 (6), p. 1708-1715-
dc.contributor.authorscopusid7006442271-
dc.contributor.authorscopusid36838126500-
dc.contributor.authorscopusid25628359100-
dc.contributor.authorscopusid30567472900-
dc.contributor.authorscopusid25627673000-
dc.contributor.authorscopusid55617275900-
dc.contributor.authorscopusid6506380646-
dc.contributor.authorscopusid7005215846-
dc.contributor.authorscopusid7004323423-
dc.description.lastpage1715en_US
dc.description.firstpage1708en_US
dc.relation.volume110en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000291532600028-
dc.contributor.daisngid909211-
dc.contributor.daisngid362523-
dc.contributor.daisngid7526316-
dc.contributor.daisngid1264657-
dc.contributor.daisngid1193189-
dc.contributor.daisngid861361-
dc.contributor.daisngid31470516-
dc.contributor.daisngid1230811-
dc.contributor.daisngid5373905-
dc.contributor.daisngid33798-
dc.contributor.daisngid90295-
dc.identifier.investigatorRIDH-4961-2016-
dc.identifier.investigatorRIDH-9500-2015-
dc.identifier.investigatorRIDH-6693-2015-
dc.identifier.investigatorRIDH-6911-2018-
dc.identifier.investigatorRIDG-9739-2015-
dc.description.numberofpages8en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Guerra, B-
dc.contributor.wosstandardWOS:Gomez-Cabrera, MC-
dc.contributor.wosstandardWOS:Ponce-Gonzalez, JG-
dc.contributor.wosstandardWOS:Martinez-Bello, VE-
dc.contributor.wosstandardWOS:Guadalupe-Grau, A-
dc.contributor.wosstandardWOS:Santana, A-
dc.contributor.wosstandardWOS:Sebastia, V-
dc.contributor.wosstandardWOS:Vina, J-
dc.contributor.wosstandardWOS:Calbet, JAL-
dc.date.coverdateJunio 2011en_US
dc.identifier.ulpgcen_US
dc.description.sjr1,662
dc.description.jcr3,753
dc.description.sjrqQ2
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptFarmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptCiencias Clínicas-
crisitem.author.deptCiencias Clínicas-
crisitem.author.deptRendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptEducación Física-
crisitem.author.orcid0000-0003-4355-5682-
crisitem.author.orcid0000-0002-9215-6234-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameGuerra Hernández, Carlos Borja-
crisitem.author.fullNameSantana Rodríguez, Alfredo-
crisitem.author.fullNameLópez Calbet, José Antonio-
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