Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/46089
Título: Phenotypic and functional characterization of glucagon-positive cells derived from spontaneous differentiation of D3-mouse embryonic stem cells
Autores/as: Vicente-Salar, Nestor
Santana, Alfredo 
Juan-Picó, Pablo
Reig, Juan A.
Roche, Enrique
Clasificación UNESCO: 32 Ciencias médicas
241003 Citología humana
Palabras clave: Cell culture
Definitive endoderm
Neuroectoderm
Pancreatic hormones
Stem cell
Fecha de publicación: 2013
Publicación seriada: Cytotherapy 
Resumen: Background: Glucagon expression is being considered as a definitive endoderm marker in protocols aiming to obtain insulin-secreting cells from embryonic stem cells. However, it should be considered that in vivo glucagon is expressed both in definitive endoderm- and neuroectoderm-derived cells. Therefore, the true nature and function of in vitro spontaneously differentiated glucagon-positive cells remains to be established. Methods: D3 and R1 mouse embryonic stem cells as well as α-TC1-9 cells were cultured and glucagon expression was determined by real-time PCR and immunocytochemistry. Functional analyses regarding intracellular calcium oscillations were performed to further characterize glucagon(+) cells. Results: Specifically, 5% of D3 and R1 cells expressed preproglucagon, with a small percentage of these (<1%) expressing glucagon-like peptide 1. The constitutive expression of protein convertase 5 supports the expression of both peptides. Glucagon(+) cells co-expressed neurofilament middle and some glucagon-like peptide-1(+) cells, glial fibrillary acidic protein, indicating a neuroectodermic origin. However, few glucagon-like peptide-1(+) cells did not show coexpression with glial fibrillary acidic protein, suggesting a non-neuroectodermic origin for these cells. Finally, glucagon(+) cells did not display Ca(2+) oscillations typical of pancreatic α-cells. Discussion: These results indicate the possible nondefinitive endodermal origin of glucagon-positive cells spontaneously differentiated from D3 and R1 cell lines, as well as the presence of cells expressing glucagon-like peptide-1 from two different origins.
URI: http://hdl.handle.net/10553/46089
ISSN: 1465-3249
DOI: 10.1016/j.jcyt.2012.08.002
Fuente: Cytotherapy [ISSN 1465-3249], v. 15, p. 122-131
Colección:Artículos
Vista completa

Visitas

25
actualizado el 05-ago-2023

Google ScholarTM

Verifica

Altmetric


Comparte



Exporta metadatos



Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor con todos los derechos reservados, a menos que se indique lo contrario.