Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/46089
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dc.contributor.authorVicente-Salar, Nestoren_US
dc.contributor.authorSantana, Alfredoen_US
dc.contributor.authorJuan-Picó, Pabloen_US
dc.contributor.authorReig, Juan A.en_US
dc.contributor.authorRoche, Enriqueen_US
dc.date.accessioned2018-11-23T01:16:22Z-
dc.date.available2018-11-23T01:16:22Z-
dc.date.issued2013en_US
dc.identifier.issn1465-3249en_US
dc.identifier.urihttp://hdl.handle.net/10553/46089-
dc.description.abstractBackground: Glucagon expression is being considered as a definitive endoderm marker in protocols aiming to obtain insulin-secreting cells from embryonic stem cells. However, it should be considered that in vivo glucagon is expressed both in definitive endoderm- and neuroectoderm-derived cells. Therefore, the true nature and function of in vitro spontaneously differentiated glucagon-positive cells remains to be established. Methods: D3 and R1 mouse embryonic stem cells as well as α-TC1-9 cells were cultured and glucagon expression was determined by real-time PCR and immunocytochemistry. Functional analyses regarding intracellular calcium oscillations were performed to further characterize glucagon(+) cells. Results: Specifically, 5% of D3 and R1 cells expressed preproglucagon, with a small percentage of these (<1%) expressing glucagon-like peptide 1. The constitutive expression of protein convertase 5 supports the expression of both peptides. Glucagon(+) cells co-expressed neurofilament middle and some glucagon-like peptide-1(+) cells, glial fibrillary acidic protein, indicating a neuroectodermic origin. However, few glucagon-like peptide-1(+) cells did not show coexpression with glial fibrillary acidic protein, suggesting a non-neuroectodermic origin for these cells. Finally, glucagon(+) cells did not display Ca(2+) oscillations typical of pancreatic α-cells. Discussion: These results indicate the possible nondefinitive endodermal origin of glucagon-positive cells spontaneously differentiated from D3 and R1 cell lines, as well as the presence of cells expressing glucagon-like peptide-1 from two different origins.en_US
dc.languageengen_US
dc.relation.ispartofCytotherapyen_US
dc.sourceCytotherapy [ISSN 1465-3249], v. 15, p. 122-131en_US
dc.subject32 Ciencias médicasen_US
dc.subject241003 Citología humanaen_US
dc.subject.otherCell cultureen_US
dc.subject.otherDefinitive endodermen_US
dc.subject.otherNeuroectodermen_US
dc.subject.otherPancreatic hormonesen_US
dc.subject.otherStem cellen_US
dc.titlePhenotypic and functional characterization of glucagon-positive cells derived from spontaneous differentiation of D3-mouse embryonic stem cellsen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.jcyt.2012.08.002en_US
dc.identifier.scopus84875414018-
dc.contributor.authorscopusid15053628100-
dc.contributor.authorscopusid55617275900-
dc.contributor.authorscopusid10440534300-
dc.contributor.authorscopusid7006825116-
dc.contributor.authorscopusid7005472886-
dc.description.lastpage131en_US
dc.description.firstpage122en_US
dc.relation.volume15en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.description.numberofpages10en_US
dc.utils.revisionen_US
dc.date.coverdateEnero 2013en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr1,17
dc.description.jcr3,1
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptCiencias Clínicas-
crisitem.author.fullNameSantana Rodríguez, Alfredo-
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