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Title: R1881 regulation of steroidogenesis in cultured testicular cells
Authors: Fanjul, L. F. 
Estevez, F. 
Deniz, A.
Marrero, I. 
Benitez, L.
Quintana, J 
Gonzalez, J.
Centol, I.
Ruiz de Galarreta, C. M.
UNESCO Clasification: 32 Ciencias médicas
2403 Bioquímica
Keywords: R1881
Testicular cells
Issue Date: 1989
Journal: Biochemistry International 
Abstract: The influence of a synthetic androgen R1881 upon hCG stimulated steroidogenesis in cultured rat testicular cells was investigated. Testicular cells were cultured for 8 days in medium alone and thereafter reincubated for 48 h with appropriate treatments before the collection of media for steroid RIA. Addition of R1881 (10(-6) M) resulted in an overall decrease of hCG (0.3-10 ng/ml) stimulated pregnenolone and progesterone production by cultured cells. The conversion of exogenous steroids of the delta 4 pathway (progesterone,17 alpha-OH-P and delta 4-A) was also studied in cultures supplemented with cyanoketone (10(-5) M) and/or spironolactone (10(-5) M) to prevent endogenous testosterone production. R1881 inhibited progesterone and 17 alpha-OH-P conversion to testosterone (T) and was ineffective when delta 4-A served as precursor for T biosynthesis. The inhibitory effect of R1881 upon Testosterone production was prevented by concomitant treatment with CPA. These observations suggest that R1881 decreases the hCG stimulated testosterone production via inhibition of CSCCE,3 beta-HSD,C17-20 Lyase and likely 17 alpha-Hydroxylase, whereas no effect on 17 beta-HSD could be observed.
ISSN: 0158-5231
Source: Biochemistry International [ISSN 0158-5231], v. 19, p. 301-312
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