|Title:||Taurolithocholate and taurolithocholate 3-sulphate exert different effects on cytosolic free Ca2+ concentration in rat hepatocytes||Authors:||Marrero Arencibia, María Isabel
Cobbold, P. H.
Dixon, C. J.
|UNESCO Clasification:||32 Ciencias médicas
|Issue Date:||1994||Journal:||Biochemical Journal||Abstract:||Single rat hepatocytes show repetitive oscillations in cytosolic free Ca2+ concentration ([Ca2+]i) when stimulated by agonists acting through the phosphoinositide signalling pathway. We have studied the effect of a natural bile acid, taurolithocholate (TLC), and its sulphated form, taurolithocholate 3-sulphate (TLC-S), on [Ca2+]i in single isolated rat hepatocytes. Although these bile acids are believed to act through a common mechanism to permeabilize the intracellular Ca2+ pool, the [Ca2+]i responses induced by the two compounds were different. Whereas TLC induced a sustained elevation of [Ca2+]i, TLC-S evoked repetitive [Ca2+]i oscillations. In addition, we show that ryanodine, which blocks the Ca(2+)-induced Ca2+ release ('CICR') mechanism, blocked TLC-S-induced oscillations in 50% of hepatocytes, but did not affect the TLC-induced rise in [Ca2+]i.||URI:||http://hdl.handle.net/10553/45309||ISSN:||0264-6021||DOI:||10.1042/bj3000383||Source:||Biochemical Journal [ISSN 0264-6021], v. 300, p. 383-386|
|Appears in Collections:||Artículos|
checked on Nov 27, 2022
checked on Oct 1, 2022
Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.