Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/45309
Title: | Taurolithocholate and taurolithocholate 3-sulphate exert different effects on cytosolic free Ca2+ concentration in rat hepatocytes | Authors: | Marrero Arencibia, María Isabel Sanchez-Bueno, A. Cobbold, P. H. Dixon, C. J. |
UNESCO Clasification: | 32 Ciencias médicas 320502 Endocrinología |
Keywords: | Taurolithocholate Taurolithocholate 3-sulphate Cytosolic Rat hepatocytes |
Issue Date: | 1994 | Journal: | Biochemical Journal | Abstract: | Single rat hepatocytes show repetitive oscillations in cytosolic free Ca2+ concentration ([Ca2+]i) when stimulated by agonists acting through the phosphoinositide signalling pathway. We have studied the effect of a natural bile acid, taurolithocholate (TLC), and its sulphated form, taurolithocholate 3-sulphate (TLC-S), on [Ca2+]i in single isolated rat hepatocytes. Although these bile acids are believed to act through a common mechanism to permeabilize the intracellular Ca2+ pool, the [Ca2+]i responses induced by the two compounds were different. Whereas TLC induced a sustained elevation of [Ca2+]i, TLC-S evoked repetitive [Ca2+]i oscillations. In addition, we show that ryanodine, which blocks the Ca(2+)-induced Ca2+ release ('CICR') mechanism, blocked TLC-S-induced oscillations in 50% of hepatocytes, but did not affect the TLC-induced rise in [Ca2+]i. | URI: | http://hdl.handle.net/10553/45309 | ISSN: | 0264-6021 | DOI: | 10.1042/bj3000383 | Source: | Biochemical Journal [ISSN 0264-6021], v. 300, p. 383-386 |
Appears in Collections: | Artículos |
SCOPUSTM
Citations
20
checked on Mar 30, 2025
WEB OF SCIENCETM
Citations
20
checked on Mar 30, 2025
Page view(s)
40
checked on Feb 24, 2024
Google ScholarTM
Check
Altmetric
Share
Export metadata
Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.