Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/44922
Título: Modulation of the Expression of Components of the Stress Response by Dietary Arachidonic Acid in European Sea Bass (Dicentrarchus labrax) Larvae
Autores/as: Montero, Daniel 
Terova, Genciana
Rimoldi, Simona
Betancor, Mónica B.
Atalah, Eyad
Torrecillas, Silvia
Caballero, María J. 
Zamorano, María J. 
Izquierdo, Marisol 
Palabras clave: Polyunsaturated Fatty-Acids
Bream Sparus-Aurata
Messenger-Rna Expression
Heat-Shock Proteins
Growth-Factor-I, et al.
Fecha de publicación: 2015
Editor/a: 0024-4201
Proyectos: Advanced Research Initiatives For Nutrition & Aquaculture 
Publicación seriada: Lipids 
Resumen: This study reports for the first time on European sea bass, Dicentrarchus labrax (L.), larvae, the effect of different levels of dietary arachidonic acid (ARA; 20:4n-6) on the expression of genes related to the fish stress response. Copies of mRNA from genes related to steroidogenesis [StAR (steroidogenic acute regulatory protein), c-Fos, and CYP11 beta (11 beta-hydroxylase gene)], glucocorticoid receptor complex [GR (glucocorticoid receptor) and HSP (heat shock proteins) 70 and 90) and antioxidative stress (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase] were quantified. Eighteen day-old larvae were fed for 14 days with three experimental diets with increasing levels of ARA (0.3, 0.6 and 1.2 % d.w.) and similar levels of docosahexaenoic (22:6n-3) and eicosapentaenoic (20:5n-3) acids (5 and 3 %, respectively). The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real time RT-PCR with the standard curve method (absolute quantification). Increase dietary levels of ARA induced a significantly (p < 0.05) down-regulation of genes related to cortisol synthesis, such as StAR and CYP11 beta and up-regulated genes related to glucocorticoid receptor complex, such as HSP70 and GR. No effects were observed on antioxidant enzymes gene expression. These results revealed the regulatory role of dietary ARA on the expression of stress-related genes in European sea bass larvae.
URI: http://hdl.handle.net/10553/44922
ISSN: 0024-4201
DOI: 10.1007/s11745-015-4057-1
Fuente: Lipids [ISSN 0024-4201], v. 50, p. 1029-1041
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