Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/44483
Title: SIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: Influence of glucose ingestion
Authors: Guerra, Borja 
Guadalupe Grau, Amelia 
Fuentes, Teresa 
Ponce-González, Jesús Gustavo
Morales Alamo, David 
Olmedillas, Hugo
Guillén Salgado, José Ángel 
Santana Rodríguez, Alfredo 
Calbet, Jose A. L. 
UNESCO Clasification: 241106 Fisiología del ejercicio
Keywords: Fatigue, Human, Performance, Signaling, Skeletal muscle, Wingate
Issue Date: 2010
Publisher: 1439-6319
Project: Mecanismos Fisiológicos y Moleculares de la Reducción de Masa Grasa en Seres Humanos Mediante Ejercicios de Musculación. 
Journal: European Journal of Applied Physiology 
Abstract: This study was designed to examine potential in vivo mechanisms of AMP-activated protein kinase (AMPK) phosphorylation inhibition and its downstream signaling consequences during the recovery period after a single bout of sprint exercise. Sprint exercise induces Thr172-AMPK phosphorylation and increased PGC-1α mRNA, by an unknown mechanism. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before exercising to inhibit AMPKα phosphorylation. Exercise elicited different patterns of Ser221-ACCβ, Ser473-Akt and Thr 642-AS160 phosphorylation, during the recovery period after glucose ingestion. Thirty minutes after the control sprint, Ser485AMPKα 1/Ser491-AMPKα2 phosphorylation was reduced by 33% coinciding with increased Thr172-AMPKα phosphorylation (both, P < 0.05). Glucose abolished the 30-min Thr172-AMPKα phosphorylation. Ser221-ACCβ phosphorylation was elevated immediately following and 30 min after exercise in C and G, implying a dissociation between Thr172-AMPKα and Ser221- ACCβ phosphorylation. Two hours after the sprint, PGC-1α protein expression remained unchanged while SIRT1 (its upstream deacetylase) was increased. Glucose ingestion abolished the SIRT1 response without any significant effect on PGC-1α protein expression. In conclusion, glucose ingestion prior to a sprint exercise profoundly affects Thr172- AMPKα phosphorylation and its downstream signaling during the recovery period.
URI: http://hdl.handle.net/10553/44483
ISSN: 1439-6319
DOI: 10.1007/s00421-010-1413-y
Source: European Journal Of Applied Physiology[ISSN 1439-6319],v. 109 (4), p. 731-743
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

51
checked on Feb 21, 2021

WEB OF SCIENCETM
Citations

53
checked on Feb 21, 2021

Page view(s)

17
checked on Feb 21, 2021

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.