Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/44483
DC FieldValueLanguage
dc.contributor.authorGuerra, Borjaen_US
dc.contributor.authorGuadalupe Grau, Ameliaen_US
dc.contributor.authorFuentes, Teresaen_US
dc.contributor.authorPonce-González, Jesús Gustavoen_US
dc.contributor.authorMorales Alamo, Daviden_US
dc.contributor.authorOlmedillas, Hugoen_US
dc.contributor.authorGuillén Salgado, José Ángelen_US
dc.contributor.authorSantana Rodríguez, Alfredoen_US
dc.contributor.authorCalbet, Jose A. L.en_US
dc.contributor.otherGuerra, Borja-
dc.contributor.otherCalbet, Jose A-
dc.contributor.otherGuadalupe-Grau, Amelia-
dc.contributor.otherPonce Gonzalez, Jesus Gustavo-
dc.contributor.otherMorales-Alamo, David-
dc.contributor.otherFuentes, Teresa-
dc.contributor.otherOlmedillas, Hugo-
dc.date.accessioned2018-11-21T23:57:02Z-
dc.date.available2018-11-21T23:57:02Z-
dc.date.issued2010en_US
dc.identifier.issn1439-6319en_US
dc.identifier.urihttp://hdl.handle.net/10553/44483-
dc.description.abstractThis study was designed to examine potential in vivo mechanisms of AMP-activated protein kinase (AMPK) phosphorylation inhibition and its downstream signaling consequences during the recovery period after a single bout of sprint exercise. Sprint exercise induces Thr172-AMPK phosphorylation and increased PGC-1α mRNA, by an unknown mechanism. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before exercising to inhibit AMPKα phosphorylation. Exercise elicited different patterns of Ser221-ACCβ, Ser473-Akt and Thr 642-AS160 phosphorylation, during the recovery period after glucose ingestion. Thirty minutes after the control sprint, Ser485AMPKα 1/Ser491-AMPKα2 phosphorylation was reduced by 33% coinciding with increased Thr172-AMPKα phosphorylation (both, P < 0.05). Glucose abolished the 30-min Thr172-AMPKα phosphorylation. Ser221-ACCβ phosphorylation was elevated immediately following and 30 min after exercise in C and G, implying a dissociation between Thr172-AMPKα and Ser221- ACCβ phosphorylation. Two hours after the sprint, PGC-1α protein expression remained unchanged while SIRT1 (its upstream deacetylase) was increased. Glucose ingestion abolished the SIRT1 response without any significant effect on PGC-1α protein expression. In conclusion, glucose ingestion prior to a sprint exercise profoundly affects Thr172- AMPKα phosphorylation and its downstream signaling during the recovery period.en_US
dc.languageengen_US
dc.publisher1439-6319-
dc.relationMecanismos Fisiológicos y Moleculares de la Reducción de Masa Grasa en Seres Humanos Mediante Ejercicios de Musculación.en_US
dc.relation.ispartofEuropean Journal of Applied Physiologyen_US
dc.sourceEuropean Journal Of Applied Physiology[ISSN 1439-6319],v. 109 (4), p. 731-743en_US
dc.subject241106 Fisiología del ejercicioen_US
dc.subject.otherFatigue, Human, Performance, Signaling, Skeletal muscle, Wingateen_US
dc.titleSIRT1, AMP-activated protein kinase phosphorylation and downstream kinases in response to a single bout of sprint exercise: Influence of glucose ingestionen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/s00421-010-1413-yen_US
dc.identifier.scopus77954887503-
dc.identifier.isi000278683100017-
dcterms.isPartOfEuropean Journal Of Applied Physiology-
dcterms.sourceEuropean Journal Of Applied Physiology[ISSN 1439-6319],v. 109 (4), p. 731-743-
dc.contributor.authorscopusid7006442271-
dc.contributor.authorscopusid25627673000-
dc.contributor.authorscopusid16301115800-
dc.contributor.authorscopusid25628359100-
dc.contributor.authorscopusid35148038500-
dc.contributor.authorscopusid23051517000-
dc.contributor.authorscopusid35726596300-
dc.contributor.authorscopusid57192957894-
dc.contributor.authorscopusid55617275900-
dc.contributor.authorscopusid7004323423-
dc.description.lastpage743en_US
dc.description.firstpage731en_US
dc.relation.volume109en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.wosWOS:000278683100017-
dc.contributor.daisngid909211-
dc.contributor.daisngid861361-
dc.contributor.daisngid1500209-
dc.contributor.daisngid7526316-
dc.contributor.daisngid1264657-
dc.contributor.daisngid1232764-
dc.contributor.daisngid954582-
dc.contributor.daisngid5734696-
dc.contributor.daisngid19198365-
dc.contributor.daisngid31470516-
dc.contributor.daisngid1230811-
dc.contributor.daisngid90295-
dc.identifier.investigatorRIDG-9739-2015-
dc.identifier.investigatorRIDH-6693-2015-
dc.identifier.investigatorRIDH-4961-2016-
dc.identifier.investigatorRIDH-9500-2015-
dc.identifier.investigatorRIDD-2683-2009-
dc.identifier.investigatorRIDK-7227-2014-
dc.identifier.investigatorRIDNo ID-
dc.contributor.wosstandardWOS:Guerra, B-
dc.contributor.wosstandardWOS:Guadalupe-Grau, A-
dc.contributor.wosstandardWOS:Fuentes, T-
dc.contributor.wosstandardWOS:Ponce-Gonzalez, JG-
dc.contributor.wosstandardWOS:Morales-Alamo, D-
dc.contributor.wosstandardWOS:Olmedillas, H-
dc.contributor.wosstandardWOS:Guillen-Salgado, J-
dc.contributor.wosstandardWOS:Santana, A-
dc.contributor.wosstandardWOS:Calbet, JAL-
dc.date.coverdateJulio 2010en_US
dc.identifier.ulpgces
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptFarmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptRendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Educación Física-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptRendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Educación Física-
crisitem.author.orcid0000-0003-4355-5682-
crisitem.author.orcid0000-0003-1286-8731-
crisitem.author.orcid0000-0002-9215-6234-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameGuerra Hernández, Carlos Borja-
crisitem.author.fullNameGuadalupe Grau, Amelia-
crisitem.author.fullNameHernández Delgado, Teresa De Jesús-
crisitem.author.fullNameMorales Álamo, David-
crisitem.author.fullNameGuillén Salgado, José Ángel-
crisitem.author.fullNameSantana Rodríguez, Alfredo-
crisitem.author.fullNameLópez Calbet, José Antonio-
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