Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/44481
Title: | Is sprint exercise a leptin signaling mimetic in human skeletal muscle? | Authors: | Guerra, Borja Olmedillas, Hugo Guadalupe Grau, Amelia Ponce-González, Jesús G. Morales Alamo, David Fuentes, Teresa Chapinal, Esther Fernández-Pérez, Leandro De Pablos Velasco, Pedro Luis Santana Rodríguez, Alfredo Calbet, Jose A. L. |
UNESCO Clasification: | 241106 Fisiología del ejercicio | Keywords: | IL-6 SOCS3 STAT3 p38 MAPK |
Issue Date: | 2011 | Project: | Influencia Del Polimorfismo Del Gen Del Receptor de la Vitamina D y Del Fenotipo Muscular en la Respuesta Osteogénica Al Ejercicio Con y Sin Impactos en Seres Humanos. Influencia Del Ejercicio Físico en Los Mecanismos de Señalización de Leptina en El Músculo Esquelético Humano. Mecanismos Fisiológicos y Moleculares de la Reducción de Masa Grasa en Seres Humanos Mediante Ejercicios de Musculación. Integracion de Los Grupos de la Obesidad y El Síndrome Metabólico .... |
Journal: | Journal of Applied Physiology | Abstract: | This study was designed to determine whether sprint exercise activates signaling cascades linked to leptin actions in human skeletal muscle and how this pattern of activation may be interfered by glucose ingestion. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before the Wingate test. Exercise elicited different patterns of JAK2, STAT3, STAT5, ERK1/2, p38 MAPK phosphorylation, and SOCS3 protein expression during the recovery period after glucose ingestion. Thirty minutes after the control sprint, STAT3 and ERK1/2 phosphorylation levels were augmented (both, P < 0.05). SOCS3 protein expression was increased 120 min after the control sprint but PTP1B protein expression was unaffected. Thirty and 120 min after the control sprint, STAT5 phosphorylation was augmented (P < 0.05). Glucose abolished the 30 min STAT3 and ERK1/2 phosphorylation and the 120 min SOCS3 protein expression increase while retarding the STAT5 phosphorylation response to sprint. Activation of these signaling cascades occurred despite a reduction of circulating leptin concentration after the sprint. Basal JAK2 and p38 MAPK phosphorylation levels were reduced and increased (both P < 0.05), respectively, by glucose ingestion prior to exercise. During recovery, JAK2 phosphorylation was unchanged and p38 MAPK phosphorylation was transiently reduced when the exercise was preceded by glucose ingestion. In conclusion, sprint exercise performed under fasting conditions is a leptin signaling mimetic in human skeletal muscle. | URI: | http://hdl.handle.net/10553/44481 | ISSN: | 8750-7587 | DOI: | 10.1152/japplphysiol.00805.2010 | Source: | Journal Of Applied Physiology [ISSN 8750-7587],v. 111 (3), p. 715-725, (Septiembre 2011) |
Appears in Collections: | Artículos |
Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.