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Title: GABAA and GABAB sites in bovine adrenal medulla membranes
Authors: Castro López-Tarruella, Enrique 
Oset‐Gasque, M. J.
Cañadas, S.
Gimenez, G.
González, M. P.
UNESCO Clasification: 320502 Endocrinología
240104 Citología animal
241003 Citología humana
Keywords: GABA receptor
Adrenal medulla
Issue Date: 1988
Publisher: 0360-4012
Journal: Journal of Neuroscience Research 
Abstract: The effect of several ligands and Ca2+ ions on [3H]GABA binding to bovine adrenal medulla membranes was investigated. Without any blockade, the [3H]GABA binding showed two components, one of low affinity (Kd = 139 ± 22 nM and Bmax = 3.2 ± 0.4 pmol/mg protein) and the other of high affinity (Kd = 41 ± 6 nM and Bmax = 0.35 ± 0.26 pmol/mg protein). Muscimol specifically blocked low‐affinity sites, and ( – )baclofen blocked high‐affinity components. Ca2+ ions were strictly necessary for maximum binding to high‐affinity sites, whereas they did not significantly affect sites of the lower affinity. These results show that the bovine adrenal medulla has a GABAA receptor population of low affinity together with a GABAB receptor of high affinity.
ISSN: 0360-4012
DOI: 10.1002/jnr.490200213
Source: Journal of Neuroscience Research [ISSN 0360-4012], v. 20, p. 241-245
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