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Title: Role of CD4 and CD8 T-lymphocytes, B-lymphocytes and Natural Killer cells in the prediction of radiation-induced late toxicity in cervical cancer patients
Authors: Bordón Rodríguez, Elisa de los Reyes 
Henríquez Hernández, Luis Alberto 
Lara, Pedro Carlos
Pinar, Beatriz
Rodríaguez-Gallego, Carlos
Lloret Sáez-Bravo, Marta 
UNESCO Clasification: 32 Ciencias médicas
Keywords: Late toxicity
Cervical cancer
Radiation-induced apoptosis
Issue Date: 2011
Publisher: 0955-3002
Journal: International Journal of Radiation Biology 
Abstract: Purpose: To analyse the role of in vitro radio-induced apoptosis of lymphocyte subpopulations as predictive test for late effects in cervical cancer patients treated with radiotherapy. Methods and materials: Ninety-four consecutive patients and four healthy controls were included in the study. Toxicity was evaluated using the Late Effects Normal Tissue-Subjective, Objective, Management, and Analytic (LENT-SOMA) scale. Peripheral blood lymphocyte subpopulations were isolated and irradiated at 0, 1, 2 and 8 Gy, and then collected 24, 48 and 72 h after irradiation. Apoptosis was measured by flow cytometry. Results: Radiation-induced apoptosis increased with radiation dose and time of incubation, and data fitted to a semi-logarithmic model defined by two constants: α (percentage of spontaneous cell death) and β (percentage of cell death induced at a determined radiation dose). Higher β values in cytotoxic T-lymphocytes (CD8) and bone cells (B-lymphocytes) were observed in patients with low bowel toxicity (hazard ratio (HR) = 0.96, p = 0.002 for B-cells); low rectal toxicity (HR = 0.96, p = 0.020; HR = 0.93, p = 0.05 for B and CD8 subpopulations respectively); low urinary toxicity (HR = 0.93, p = 0.003 for B-cells) and low sexual toxicity (HR = 0.93, p = 0.010 for CD8-cells). Conclusions: Radiation-induced CD8 T-lymphocytes and, for the first time, B-lymphocytes apoptosis can predict differences in late toxicity in cervical cancer patients.
ISSN: 0955-3002
DOI: 10.3109/09553002.2010.537433
Source: International Journal of Radiation Biology [ISSN 0955-3002], v. 87, p. 424-431
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