Pressurized liquid extraction of pigments from Chlamydomonas sp. and chemical characterization by HPLC-MS/MS

Title:	Pressurized liquid extraction of pigments from Chlamydomonas sp. and chemical characterization by HPLC-MS/MS
Authors:	Montero, Lidia Sedghi, Mahshid García, Yolanda Almeida, Carlos Safi, Carl Engelen-Smit, Nicole Cifuentes, Alejandro Mendiola, Jose A. Ibáñez, Elena
UNESCO Clasification:	2510 Oceanografía
Keywords:	Antioxidant capacity Chlamydomonas sp. HPLC–DAD–APCI–MS/MS Pigments Pressurized liquid extraction, et al
Issue Date:	2018
Publisher:	2096-241X
Journal:	Journal Of Analysis And Testing
Abstract:	Pressurized liquid extraction using green solvents (ethanol and water) was applied to extract photosynthetic pigments from the microalgae Chlamydomonas sp. An experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC tests. All the extracts show significant antioxidant activity in all assays. Besides, in this work, the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC-DAD-APCI-MS/MS for the first time, being the main compounds lutein and pheophythin a '.
URI:	http://hdl.handle.net/10553/42329
ISSN:	2096-241X
DOI:	10.1007/s41664-018-0062-8
Source:	Journal of Analysis and Testing [ISSN 2096-241X], v. 2 (2), p. 149-157
Appears in Collections:	Artículos

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