Please use this identifier to cite or link to this item:
Title: Airway transplantation of adipose stem cells protects against bleomycin-induced pulmonary fibrosis
Authors: Llontop, Pedro
Lopez-Fernandez, Daniel 
Clavo, Bernardino 
Afonso Martin, Juan Luis
Fiuza-Perez, Maria D. 
García Arranz, Mariano
Calatayud, Joaquín
Molins López-Rodó, Laureano
Alshehri, Khalid
Ayub, Adil
Raad, Wissam
Bhora, Faiz
Santana-Rodriguez, Norberto
UNESCO Clasification: 320508 Enfermedades pulmonares
Keywords: Nf-Kappa-B
Transcription Factor
Model, et al
Issue Date: 2018
Journal: Journal of Investigative Medicine 
Abstract: Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with poor prognosis. Adipose-derived stem cells (ADSC) have demonstrated regenerative properties in several tissues. The hypothesis of this study was that airway transplantation of ADSC could protect against bleomycin (BLM)-induced pulmonary fibrosis (PF). Fifty-eight lungs from 29 male Sprague-Dawley rats were analyzed. Animals were randomly divided into five groups: a) control (n=3); b) sham (n=6); c) BLM (n=6); d) BLM+ADSC-2d (n=6); and e) BLM+ADSC-14d (n=8). Animals received 500 mu L saline (sham), 2.5 UI/kg BLM in 500 mu L saline (BLM), and 2x10(6)ADSC in 100 mu L saline intratracheally at 2 (BLM+ADSC-2d) and 14 days (BLM+ADSC-14d) after BLM. Animals were sacrificed at 28 days. Blinded Ashcroft score was used to determine pulmonary fibrosis extent on histology. Hsp27, Vegf, Nfk, IL-1, IL-6, Col4, and Tgf1 mRNA gene expression were determined using real-time quantitative-PCR. Ashcroft index was: control=0; sham=0.37 +/- 0.07; BLM=6.55 +/- 0.34vs sham (P=0.006). BLM vs BLM+ADSC-2d=4.63 +/- 0.38 (P=0.005) and BLM+ADSC-14d=3.77 +/- 0.46 (P=0.005). BLM vs sham significantly increased Hsp27 (P=0.018), Nfk (P=0.009), Col4 (P=0.004), Tgf1 (P=0.006) and decreased IL-1 (P=0.006). BLM+ADSC-2d vs BLM significantly decreased Hsp27 (P=0.009) and increased Vegf (P=0.006), Nfk (P=0.009). BLM+ADSC-14d vs BLM significantly decreased Hsp27 (P=0.028), IL-6 (P=0.013), Col4 (P=0.002), and increased Nfk (P=0.040) and Tgf1 (P=0.002). Airway transplantation of ADSC significantly decreased the fibrosis rate in both early and established pulmonary fibrosis, modulating the expression of Hsp27, Vegfa, Nfk, IL-6, Col4, and Tgf1. From a translational perspective, this technique could become a new adjuvant treatment for patients with IPF.
ISSN: 1081-5589
DOI: 10.1136/jim-2017-000494
Source: Journal Of Investigative Medicine[ISSN 1081-5589],v. 66 (4), p. 739-746
Appears in Collections:Artículos
Show full item record


checked on May 26, 2024


checked on May 26, 2024

Page view(s)

checked on Jan 27, 2024

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.