Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/41743
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Llontop, Pedro | en_US |
dc.contributor.author | Lopez-Fernandez, Daniel | en_US |
dc.contributor.author | Clavo, Bernardino | en_US |
dc.contributor.author | Afonso Martin, Juan Luis | en_US |
dc.contributor.author | Fiuza-Perez, Maria D. | en_US |
dc.contributor.author | García Arranz, Mariano | en_US |
dc.contributor.author | Calatayud, Joaquín | en_US |
dc.contributor.author | Molins López-Rodó, Laureano | en_US |
dc.contributor.author | Alshehri, Khalid | en_US |
dc.contributor.author | Ayub, Adil | en_US |
dc.contributor.author | Raad, Wissam | en_US |
dc.contributor.author | Bhora, Faiz | en_US |
dc.contributor.author | Santana-Rodriguez, Norberto | en_US |
dc.contributor.other | LOPEZ FERNANDEZ, DANIEL | |
dc.date.accessioned | 2018-08-03T10:39:34Z | - |
dc.date.available | 2018-08-03T10:39:34Z | - |
dc.date.issued | 2018 | en_US |
dc.identifier.issn | 1081-5589 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/41743 | - |
dc.description.abstract | Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with poor prognosis. Adipose-derived stem cells (ADSC) have demonstrated regenerative properties in several tissues. The hypothesis of this study was that airway transplantation of ADSC could protect against bleomycin (BLM)-induced pulmonary fibrosis (PF). Fifty-eight lungs from 29 male Sprague-Dawley rats were analyzed. Animals were randomly divided into five groups: a) control (n=3); b) sham (n=6); c) BLM (n=6); d) BLM+ADSC-2d (n=6); and e) BLM+ADSC-14d (n=8). Animals received 500 mu L saline (sham), 2.5 UI/kg BLM in 500 mu L saline (BLM), and 2x10(6)ADSC in 100 mu L saline intratracheally at 2 (BLM+ADSC-2d) and 14 days (BLM+ADSC-14d) after BLM. Animals were sacrificed at 28 days. Blinded Ashcroft score was used to determine pulmonary fibrosis extent on histology. Hsp27, Vegf, Nfk, IL-1, IL-6, Col4, and Tgf1 mRNA gene expression were determined using real-time quantitative-PCR. Ashcroft index was: control=0; sham=0.37 +/- 0.07; BLM=6.55 +/- 0.34vs sham (P=0.006). BLM vs BLM+ADSC-2d=4.63 +/- 0.38 (P=0.005) and BLM+ADSC-14d=3.77 +/- 0.46 (P=0.005). BLM vs sham significantly increased Hsp27 (P=0.018), Nfk (P=0.009), Col4 (P=0.004), Tgf1 (P=0.006) and decreased IL-1 (P=0.006). BLM+ADSC-2d vs BLM significantly decreased Hsp27 (P=0.009) and increased Vegf (P=0.006), Nfk (P=0.009). BLM+ADSC-14d vs BLM significantly decreased Hsp27 (P=0.028), IL-6 (P=0.013), Col4 (P=0.002), and increased Nfk (P=0.040) and Tgf1 (P=0.002). Airway transplantation of ADSC significantly decreased the fibrosis rate in both early and established pulmonary fibrosis, modulating the expression of Hsp27, Vegfa, Nfk, IL-6, Col4, and Tgf1. From a translational perspective, this technique could become a new adjuvant treatment for patients with IPF. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Investigative Medicine | en_US |
dc.source | Journal Of Investigative Medicine[ISSN 1081-5589],v. 66 (4), p. 739-746 | en_US |
dc.subject | 320508 Enfermedades pulmonares | en_US |
dc.subject.other | Nf-Kappa-B | |
dc.subject.other | Transcription Factor | |
dc.subject.other | Growth-Factors | |
dc.subject.other | Lung | |
dc.subject.other | Model | |
dc.subject.other | Rat | |
dc.subject.other | Expression | |
dc.subject.other | Statement | |
dc.subject.other | Therapy | |
dc.subject.other | Disease | |
dc.title | Airway transplantation of adipose stem cells protects against bleomycin-induced pulmonary fibrosis | en_US |
dc.type | info:eu-repo/semantics/Article | es |
dc.type | Article | es |
dc.identifier.doi | 10.1136/jim-2017-000494 | |
dc.identifier.scopus | 85048876688 | |
dc.identifier.isi | 000429735000004 | |
dcterms.isPartOf | Journal Of Investigative Medicine | |
dcterms.source | Journal Of Investigative Medicine[ISSN 1081-5589],v. 66 (4), p. 739-746 | |
dc.contributor.authorscopusid | 37041705700 | |
dc.contributor.authorscopusid | 57211140324 | |
dc.contributor.authorscopusid | 56940678900 | |
dc.contributor.authorscopusid | 57190093030 | |
dc.contributor.authorscopusid | 57202600785 | |
dc.contributor.authorscopusid | 6507362808 | |
dc.contributor.authorscopusid | 56501676000 | |
dc.contributor.authorscopusid | 57202606585 | |
dc.contributor.authorscopusid | 8657676900 | |
dc.contributor.authorscopusid | 24390800100 | |
dc.contributor.authorscopusid | 56636875400 | |
dc.contributor.authorscopusid | 57190859109 | |
dc.contributor.authorscopusid | 56635224400 | |
dc.contributor.authorscopusid | 21833738100 | |
dc.contributor.authorscopusid | 56072780900 | |
dc.description.lastpage | 746 | - |
dc.identifier.issue | 4 | - |
dc.description.firstpage | 739 | - |
dc.relation.volume | 66 | - |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.identifier.wos | WOS:000429735000004 | - |
dc.contributor.daisngid | 3257911 | |
dc.contributor.daisngid | 6130827 | |
dc.contributor.daisngid | 777033 | |
dc.contributor.daisngid | 5321867 | |
dc.contributor.daisngid | 6023199 | |
dc.contributor.daisngid | 4096350 | |
dc.contributor.daisngid | 34788118 | |
dc.contributor.daisngid | 468673 | |
dc.contributor.daisngid | 3231570 | |
dc.contributor.daisngid | 4380464 | |
dc.contributor.daisngid | 922353 | |
dc.contributor.daisngid | 2520838 | |
dc.contributor.daisngid | 479353 | |
dc.contributor.daisngid | 1685892 | |
dc.identifier.investigatorRID | D-5050-2016 | |
dc.contributor.wosstandard | WOS:Llontop, P | |
dc.contributor.wosstandard | WOS:Lopez-Fernandez, D | |
dc.contributor.wosstandard | WOS:Clavo, B | |
dc.contributor.wosstandard | WOS:Martin, JLA | |
dc.contributor.wosstandard | WOS:Fiuza-Perez, MD | |
dc.contributor.wosstandard | WOS:Arranz, MG | |
dc.contributor.wosstandard | WOS:Calatayud, J | |
dc.contributor.wosstandard | WOS:Lopez-Rodo, LM | |
dc.contributor.wosstandard | WOS:Alshehri, K | |
dc.contributor.wosstandard | WOS:Ayub, A | |
dc.contributor.wosstandard | WOS:Raad, W | |
dc.contributor.wosstandard | WOS:Bhora, F | |
dc.contributor.wosstandard | WOS:Santana-Rodriguez, N | |
dc.date.coverdate | Abril 2018 | |
dc.identifier.ulpgc | Sí | es |
dc.description.sjr | 0,747 | |
dc.description.jcr | 1,994 | |
dc.description.sjrq | Q2 | |
dc.description.jcrq | Q2 | |
dc.description.scie | SCIE | |
item.fulltext | Sin texto completo | - |
item.grantfulltext | none | - |
crisitem.author.dept | Departamento de Ciencias Médicas y Quirúrgicas | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.orcid | 0000-0003-2131-9515 | - |
crisitem.author.orcid | 0000-0003-2522-1064 | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.fullName | López Fernández, Daniel | - |
crisitem.author.fullName | Clavo Varas,Bernardino | - |
crisitem.author.fullName | Fiuza Pérez,Mª Dolores | - |
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