Please use this identifier to cite or link to this item:
http://hdl.handle.net/10553/41743
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Llontop, Pedro | en_US |
dc.contributor.author | Lopez-Fernandez, Daniel | en_US |
dc.contributor.author | Clavo, Bernardino | en_US |
dc.contributor.author | Afonso Martin, Juan Luis | en_US |
dc.contributor.author | Fiuza-Perez, Maria D. | en_US |
dc.contributor.author | García Arranz, Mariano | en_US |
dc.contributor.author | Calatayud, Joaquín | en_US |
dc.contributor.author | Molins López-Rodó, Laureano | en_US |
dc.contributor.author | Alshehri, Khalid | en_US |
dc.contributor.author | Ayub, Adil | en_US |
dc.contributor.author | Raad, Wissam | en_US |
dc.contributor.author | Bhora, Faiz | en_US |
dc.contributor.author | Santana-Rodriguez, Norberto | en_US |
dc.contributor.other | LOPEZ FERNANDEZ, DANIEL | |
dc.date.accessioned | 2018-08-03T10:39:34Z | - |
dc.date.available | 2018-08-03T10:39:34Z | - |
dc.date.issued | 2018 | en_US |
dc.identifier.issn | 1081-5589 | en_US |
dc.identifier.uri | http://hdl.handle.net/10553/41743 | - |
dc.description.abstract | Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with poor prognosis. Adipose-derived stem cells (ADSC) have demonstrated regenerative properties in several tissues. The hypothesis of this study was that airway transplantation of ADSC could protect against bleomycin (BLM)-induced pulmonary fibrosis (PF). Fifty-eight lungs from 29 male Sprague-Dawley rats were analyzed. Animals were randomly divided into five groups: a) control (n=3); b) sham (n=6); c) BLM (n=6); d) BLM+ADSC-2d (n=6); and e) BLM+ADSC-14d (n=8). Animals received 500 mu L saline (sham), 2.5 UI/kg BLM in 500 mu L saline (BLM), and 2x10(6)ADSC in 100 mu L saline intratracheally at 2 (BLM+ADSC-2d) and 14 days (BLM+ADSC-14d) after BLM. Animals were sacrificed at 28 days. Blinded Ashcroft score was used to determine pulmonary fibrosis extent on histology. Hsp27, Vegf, Nfk, IL-1, IL-6, Col4, and Tgf1 mRNA gene expression were determined using real-time quantitative-PCR. Ashcroft index was: control=0; sham=0.37 +/- 0.07; BLM=6.55 +/- 0.34vs sham (P=0.006). BLM vs BLM+ADSC-2d=4.63 +/- 0.38 (P=0.005) and BLM+ADSC-14d=3.77 +/- 0.46 (P=0.005). BLM vs sham significantly increased Hsp27 (P=0.018), Nfk (P=0.009), Col4 (P=0.004), Tgf1 (P=0.006) and decreased IL-1 (P=0.006). BLM+ADSC-2d vs BLM significantly decreased Hsp27 (P=0.009) and increased Vegf (P=0.006), Nfk (P=0.009). BLM+ADSC-14d vs BLM significantly decreased Hsp27 (P=0.028), IL-6 (P=0.013), Col4 (P=0.002), and increased Nfk (P=0.040) and Tgf1 (P=0.002). Airway transplantation of ADSC significantly decreased the fibrosis rate in both early and established pulmonary fibrosis, modulating the expression of Hsp27, Vegfa, Nfk, IL-6, Col4, and Tgf1. From a translational perspective, this technique could become a new adjuvant treatment for patients with IPF. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Investigative Medicine | en_US |
dc.source | Journal Of Investigative Medicine[ISSN 1081-5589],v. 66 (4), p. 739-746 | en_US |
dc.subject | 320508 Enfermedades pulmonares | en_US |
dc.subject.other | Nf-Kappa-B | |
dc.subject.other | Transcription Factor | |
dc.subject.other | Growth-Factors | |
dc.subject.other | Lung | |
dc.subject.other | Model | |
dc.subject.other | Rat | |
dc.subject.other | Expression | |
dc.subject.other | Statement | |
dc.subject.other | Therapy | |
dc.subject.other | Disease | |
dc.title | Airway transplantation of adipose stem cells protects against bleomycin-induced pulmonary fibrosis | en_US |
dc.type | info:eu-repo/semantics/Article | es |
dc.type | Article | es |
dc.identifier.doi | 10.1136/jim-2017-000494 | |
dc.identifier.scopus | 85048876688 | |
dc.identifier.isi | 000429735000004 | |
dcterms.isPartOf | Journal Of Investigative Medicine | |
dcterms.source | Journal Of Investigative Medicine[ISSN 1081-5589],v. 66 (4), p. 739-746 | |
dc.contributor.authorscopusid | 37041705700 | |
dc.contributor.authorscopusid | 57211140324 | |
dc.contributor.authorscopusid | 56940678900 | |
dc.contributor.authorscopusid | 57190093030 | |
dc.contributor.authorscopusid | 57202600785 | |
dc.contributor.authorscopusid | 6507362808 | |
dc.contributor.authorscopusid | 56501676000 | |
dc.contributor.authorscopusid | 57202606585 | |
dc.contributor.authorscopusid | 8657676900 | |
dc.contributor.authorscopusid | 24390800100 | |
dc.contributor.authorscopusid | 56636875400 | |
dc.contributor.authorscopusid | 57190859109 | |
dc.contributor.authorscopusid | 56635224400 | |
dc.contributor.authorscopusid | 21833738100 | |
dc.contributor.authorscopusid | 56072780900 | |
dc.description.lastpage | 746 | - |
dc.identifier.issue | 4 | - |
dc.description.firstpage | 739 | - |
dc.relation.volume | 66 | - |
dc.investigacion | Ciencias de la Salud | en_US |
dc.type2 | Artículo | en_US |
dc.identifier.wos | WOS:000429735000004 | - |
dc.contributor.daisngid | 3257911 | |
dc.contributor.daisngid | 6130827 | |
dc.contributor.daisngid | 777033 | |
dc.contributor.daisngid | 5321867 | |
dc.contributor.daisngid | 6023199 | |
dc.contributor.daisngid | 4096350 | |
dc.contributor.daisngid | 34788118 | |
dc.contributor.daisngid | 468673 | |
dc.contributor.daisngid | 3231570 | |
dc.contributor.daisngid | 4380464 | |
dc.contributor.daisngid | 922353 | |
dc.contributor.daisngid | 2520838 | |
dc.contributor.daisngid | 479353 | |
dc.contributor.daisngid | 1685892 | |
dc.identifier.investigatorRID | D-5050-2016 | |
dc.contributor.wosstandard | WOS:Llontop, P | |
dc.contributor.wosstandard | WOS:Lopez-Fernandez, D | |
dc.contributor.wosstandard | WOS:Clavo, B | |
dc.contributor.wosstandard | WOS:Martin, JLA | |
dc.contributor.wosstandard | WOS:Fiuza-Perez, MD | |
dc.contributor.wosstandard | WOS:Arranz, MG | |
dc.contributor.wosstandard | WOS:Calatayud, J | |
dc.contributor.wosstandard | WOS:Lopez-Rodo, LM | |
dc.contributor.wosstandard | WOS:Alshehri, K | |
dc.contributor.wosstandard | WOS:Ayub, A | |
dc.contributor.wosstandard | WOS:Raad, W | |
dc.contributor.wosstandard | WOS:Bhora, F | |
dc.contributor.wosstandard | WOS:Santana-Rodriguez, N | |
dc.date.coverdate | Abril 2018 | |
dc.identifier.ulpgc | Sí | es |
dc.description.sjr | 0,747 | |
dc.description.jcr | 1,994 | |
dc.description.sjrq | Q2 | |
dc.description.jcrq | Q2 | |
dc.description.scie | SCIE | |
item.grantfulltext | none | - |
item.fulltext | Sin texto completo | - |
crisitem.author.dept | Departamento de Ciencias Médicas y Quirúrgicas | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.dept | GIR IUIBS: Farmacología Molecular y Traslacional | - |
crisitem.author.dept | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.orcid | 0000-0003-2131-9515 | - |
crisitem.author.orcid | 0000-0003-2522-1064 | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.parentorg | IU de Investigaciones Biomédicas y Sanitarias | - |
crisitem.author.fullName | López Fernández, Daniel | - |
crisitem.author.fullName | Clavo Varas,Bernardino | - |
crisitem.author.fullName | Fiuza Pérez,Mª Dolores | - |
Appears in Collections: | Artículos |
SCOPUSTM
Citations
12
checked on Sep 29, 2024
WEB OF SCIENCETM
Citations
9
checked on Sep 29, 2024
Page view(s)
61
checked on Jun 29, 2024
Google ScholarTM
Check
Altmetric
Share
Export metadata
Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.