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Title: Susceptibility of Malassezia pachydermatis to aminoglycosides
Authors: Silva Sergent, Freddy Alejandro 
Conde De Felipe, Magnolia María 
Rosario Medina, Mª Inmaculada 
Real Valcárcel, Fernando 
Déniz, Soraya 
Acosta, Félix 
Padilla, Daniel 
Acosta-Hernández, Begoña 
Ferrer Quintana, Otilia Rosa 
UNESCO Clasification: 3109 Ciencias veterinarias
310907 Patología
Keywords: Aminoglycosides
Malassezia, et al
Issue Date: 2017
Journal: Mycoses 
Abstract: Previous studies have evaluated the action of gentamicin against Malassezia pachydermatis. The aim of this study was to evaluate in vitro susceptibility of M. pachydermatis to the aminoglycosides— gentamicin, tobramycin, netilmicin and framycetin. The minimum inhibitory concentration (MIC) of gentamicin was determined following methods M27-A3 microdilution and Etest ® . The Etest ® was used to determine the minimum inhibitory concentration (MIC) of the tobramycin and netilmicin. The Kirby-Bauer test was used to determine the antibiotic susceptibility to the framycetin. The MIC50 and MIC90 were 8.12 μg/mL and 32.5 μg/mL by microdilution method for gentamicin. The MIC50, determined by the Etest ® , was 8 μg/mL for gentamicin and netilmicin and 64 μg/mL for tobramycin. The MIC90 was 16 and 32 μg/mL for gentamicin and netilmicin respectively. The MIC90 was outside of the detectable limits for tobramycin. To framycetin, 28 strains (40%) of the 70 M. pachydermatis isolates tested showed a diameter of 22 mm, 22 strains (31.42%) showed a diameter of 20 mm, 16 strains showed a diameter of ≤ 18 mm, and only 5.71% of the isolates showed a diameter of ≥ 22 mm. This study provides evidence of high in vitro activity of the aminoglycosides—gentamicin, tobramycin, netilmicin and framycetin against M. pachydermatis. For gentamicin Etest ® showed similar values of MIC50 y MIC90 that the obtained by microdilution method. We considered Etest ® method could be a good method for these calculations with aminoglycosides.
ISSN: 0933-7407
DOI: 10.1111/myc.12665
Source: Mycoses[ISSN 0933-7407],v. 60, p. 796-799
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