Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/21076
Title: Lipid profiling and transcriptomic analysis reveals a functional interplay between estradiol and growth hormone in liver
Authors: Fernandez-Perez, Leandro 
Santana Farré, Ruymán
Mirecki Garrido, Mercedes de 
García, Irma
Guerra, Borja 
Mateos-Díaz, Carlos
Iglesias-Gato, Diego
Carlos Diaz-Chico, Juan 
Flores Morales, Amílcar
Díaz, Mario 
UNESCO Clasification: 320502 Endocrinología
Keywords: Lipid, Liver, Growth hormone, estradiol
Issue Date: 2014
Project: Estudio Funcional y Molecular de Las Proteínas Socs en Un Modelo de Resistencia Hepática A Insulina. 
Journal: PLoS ONE 
Abstract: 17b-estradiol (E2) may interfere with endocrine, metabolic, and gender-differentiated functions in liver in both females and males. Indirect mechanisms play a crucial role because of the E2 influence on the pituitary GH secretion and the GHR-JAK2- STAT5 signaling pathway in the target tissues. E2, through its interaction with the estrogen receptor, exerts direct effects on liver. Hypothyroidism also affects endocrine and metabolic functions of the liver, rendering a metabolic phenotype with features that mimic deficiencies in E2 or GH. In this work, we combined the lipid and transcriptomic analysis to obtain comprehensive information on the molecular mechanisms of E2 effects, alone and in combination with GH, to regulate liver functions in males. We used the adult hypothyroid-orchidectomized rat model to minimize the influence of internal hormones on E2 treatment and to explore its role in male-differentiated functions. E2 influenced genes involved in metabolism of lipids and endo-xenobiotics, and the GH-regulated endocrine, metabolic, immune, and male-specific responses. E2 induced a female-pattern of gene expression and inhibited GH-regulated STAT5b targeted genes. E2 did not prevent the inhibitory effects of GH on urea and amino acid metabolism-related genes. The combination of E2 and GH decreased transcriptional immune responses. E2 decreased the hepatic content of saturated fatty acids and induced a transcriptional program that seems to be mediated by the activation of PPARa. In contrast, GH inhibited fatty acid oxidation. Both E2 and GH replacements reduced hepatic CHO levels and increased the formation of cholesterol esters and triacylglycerols. Notably, the hepatic lipid profiles were endowed with singular fingerprints that may be used to segregate the effects of different hormonal replacements. In summary, we provide in vivo evidence that E2 has a significant impact on lipid content and transcriptome in male liver and that E2 exerts a marked influence on GH physiology, with implications in human therapy.
URI: http://hdl.handle.net/10553/21076
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0096305
Source: PLoS ONE [EISSN 1932-6203], v. 9 (5), (Mayo 2014)
Rights: by-nc-nd
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