Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/123781
Título: Cetacean Intracytoplasmic Eosinophilic Globules: A Cytomorphological, Histological, Histochemical, Immunohistochemical, and Proteomic Characterization
Autores/as: Fernández Rodríguez, Antonio Jesús 
Câmara, Nakita 
Sierra Pulpillo, Eva María 
Arbelo Hernández, Manuel Antonio 
Bernaldo De Quirós Miranda, Yara 
Jepson, Paul D.
Deaville, Rob
Diaz Delgado,Josue 
Suarez Santana, Cristian Manuel 
Castro Alonso, Ayoze 
Hernández Vega, Julia Natividad 
Alves Godinho,Ana 
Clasificación UNESCO: 310907 Patología
240110 Histología animal
240119 Zoología marina
Palabras clave: Albumin
α1-antitrypsine
Bycatch
Cetacean
Fibrinogen, et al.
Fecha de publicación: 2023
Proyectos: Patología Embólica (Gaseosa/Grasa) en Cetáceos 
Publicación seriada: Animals 
Resumen: The nature, etiopathogenesis, and clinicopathologic relevance of the prevalent intracytoplasmic eosinophilic globules (IEGs) within hepatocytes of cetaceans are unknown. This study aims to evaluate the presence and characterize the IEGs in the hepatocytes of cetaceans using histochemical and immunohistochemical electron microscopy, Western blot, lectin histochemistry, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry techniques. A total of 95/115 (83%) animals (16 species) exhibited histologically evident intracytoplasmic round to oval, single to multiple, hyaline eosinophilic globules within the hepatocytes. These globules were largely PAS-positive, diastase resistant, and were immunopositive for fibrinogen (FB, 97%), albumin (Alb, 85%), and α1-antitrypsine (A1AT, 53%). The IEG positivity for FB and A1AT were correlated with live-stranding, hepatic congestion and a good nutritional status. The cetaceans lacking IEGs were consistently dead stranded and had poor body conditions. The IEGs in 36 bycaught cetaceans were, all except one, FB-positive and A1AT-negative. The IEGs exhibited morphologic and compositional variations at the ultrastructural level, suggesting various stages of development and/or etiopathogenesis(es). The glycocalyx analysis suggested an FB- and A1AT-glycosylation pattern variability between cetaceans and other animals. The proteomic analyses confirmed an association between the IEGs and acute phase proteins, suggesting a relationship between acute stress (i.e., bycatch), disease, and cellular protective mechanisms, allowing pathologists to correlate this morphological change using the acute hepatocytic cell response under certain stress conditions.
URI: http://hdl.handle.net/10553/123781
ISSN: 2076-2615
DOI: 10.3390/ani13132130
Fuente: Animals [EISSN 2076-2615],v. 13 (13), 2130, (Junio 2023)
Colección:Artículos
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