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Title: Kinetics of the invasion of a non-phagocytic fish cell line, RTG-2 by Yersinia ruckeri serotype O1 biotype 1
Authors: Padilla Castillo, Daniel Fermín 
Acosta Hernández, Begoña María 
Ramos Vivas, Jose 
Déniz Suárez, María Soraya 
Rosario Medina, Mª Inmaculada 
Martín Barrasa, José Luis 
Silva Sergent, Freddy Alejandro 
Ramos Sosa, María José 
García Álvarez, Natalia 
Real Valcárcel, Fernando 
Sánchez Henao, Julián Andrés 
UNESCO Clasification: 3105 Peces y fauna silvestre
310907 Patología
Keywords: Yersinia ruckeri
Fish disease
Cell invasion
Non-phagocytic cells
Carrier state
Issue Date: 2022
Journal: Acta Veterinaria Hungarica 
Abstract: Yersiniosis, caused by the fish pathogen Yersinia ruckeri, is a serious bacterial septicaemia affecting mainly salmonids worldwide. The acute infection may result in high mortality without apparent external disease signs, while the chronic one causes moderate to considerable mortality. Survivors of yersiniosis outbreaks become carriers. Y. ruckeri is able to adhere to, and to invade, phagocytic and non-phagocytic fish cells by using unknown molecular mechanisms. The aim of this study was to describe the kinetics of cell invasion by Y. ruckeri serotype O1 biotype 1 in a fish cell line (RTG-2) originating from rainbow trout gonads. The efficiency of invasion by Y. ruckeri was found to be temperature dependent, having a maximum at 20 °C. The bacterium was able to survive up to 96 h postinfection. The incubation of the cells at 4 °C and the pre-incubation of the bacteria with sugars or heat-inactivated antiserum significantly decreased the efficiency of invasion or even completely prevented the invasion of RTG-2 cells. These findings indicate that Y. ruckeri is capable of adhering to, entering and surviving within non-phagocytic cells, and that the intracellular environment may constitute a suitable niche for this pathogen that can favour the spread of infection and/or the maintenance of a carrier state of fish.
ISSN: 0236-6290
DOI: 10.1556/004.2022.00013
Source: Acta Veterinaria Hungarica [ISSN 0236-6290], junio 2022
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