Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/115553
Título: Oral saliva swab reverse transcription PCR for Covid-19 in the paediatric population
Autores/as: Moraleda, Cinta
Domínguez Rodríguez, Sara
Mesa, Juan Miguel
García Sánchez, Paula
de la Serna, María
Alonso Cadenas, José Antonio
Bermejo, Amanda
Sabrido, Gema
Martínez Campos, Leticia
González Posada, Aránzazu Flavia
Illán Ramos, Marta
Cobos Carrascosa, Elena
Ballesteros, Álvaro
Galán, Juan Carlos
Llorente, Francisco
Aguilera Alonso, David
Jiménez, Ana Belén
Catalán, Pilar
Dahdouh, Elias
Navarro, Ignacio
Fernández Garoz, Bárbara
Mendoza, Pablo
Pérez Jorge, Concepción
Cabezas Fernández, Teresa
Blázquez Gamero, Daniel
Rivas, Gonzalo
González Donapetry, Patricia
Sáez, Elena
Fernández Pinero, Jovita
Lucas Fernández, Jesús
Pérez Ramírez, Elisa
Merino, Paloma
Miragaya, Sandra
Lorente, Jorge
Iglesias, Irene
Tagarro, Alfredo
Peña Quintana, Luis 
Clasificación UNESCO: 32 Ciencias médicas
320110 Pediatría
320505 Enfermedades infecciosas
Palabras clave: SARS-CoV-2
PCR
Saliva test
Antigen rapid diagnostic test
Nasopharyngeal swabs
Fecha de publicación: 2022
Publicación seriada: Archives of disease in childhood 
Resumen: Objectives To evaluate the performance of oral saliva swab (OSS) reverse transcription PCR (RT-PCR) compared with RT-PCR and antigen rapid diagnostic test (Ag-RDT) on nasopharyngeal swabs (NPS) for SARS-CoV-2 in children. Design Cross-sectional multicentre diagnostic study. Setting Study nested in a prospective, observational cohort (EPICO-AEP) performed between February and March 2021 including 10 hospitals in Spain. Patients Children from 0 to 18 years with symptoms compatible with Covid-19 of ≤5 days of duration were included. Two NPS samples (Ag-RDT and RT-PCR) and one OSS sample for RT-PCR were collected. Main outcome Performance of Ag-RDT and RT-PCR on NPS and RT-PCR on OSS sample for SARS-CoV-2. Results 1174 children were included, aged 3.8 years (IQR 1.7–9.0); 73/1174 (6.2%) patients tested positive by at least one of the techniques. Sensitivity and specificity of OSS RT-PCR were 72.1% (95% CI 59.7 to 81.9) and 99.6% (95% CI 99 to 99.9), respectively, versus 61.8% (95% CI 49.1 to 73) and 99.9% (95% CI 99.4 to 100) for the Ag-RDT. Kappa index was 0.79 (95% CI 0.72 to 0.88) for OSS RT-PCR and 0.74 (95% CI 0.65 to 0.84) for Ag-RDT versus NPS RT-PCR. Conclusions RT-PCR on the OSS sample is an accurate option for SARS-CoV-2 testing in children. A less intrusive technique for younger patients, who usually are tested frequently, might increase the number of patients tested.
URI: http://hdl.handle.net/10553/115553
ISSN: 1468-2044
DOI: 10.1136/archdischild-2021-323712
Fuente: Archives of Disease in Childhood [1468-2044], p.1-8 (Junio 2022)
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