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Title: Methodology and Neuromarkers for Cetaceans’ Brains
Authors: Sacchini, Simona 
Herráez Thomas, Pedro Manuel 
Arbelo Hernández, Manuel Antonio 
Espinosa De Los Monteros Y Zayas, Antonio 
Sierra Pulpillo, Eva María 
Rivero Santana, Miguel Antonio 
Bombardi, Cristiano
Fernández Rodríguez, Antonio Jesús 
UNESCO Clasification: 3109 Ciencias veterinarias
Keywords: Beaked Whales
Methodology, et al
Issue Date: 2022
Journal: Veterinary Sciences 
Abstract: Cetacean brain sampling may be an arduous task due to the difficulty of collecting and histologically preparing such rare and large specimens. Thus, one of the main challenges of working with cetaceans’ brains is to establish a valid methodology for an optimal manipulation and fixation of the brain tissue, which allows the samples to be viable for neuroanatomical and neuropathological studies. With this in view, we validated a methodology in order to preserve the quality of such large brains (neuroanatomy/neuropathology) and at the same time to obtain fresh brain samples for toxicological, virological, and microbiological analysis (neuropathology). A fixation protocol adapted to brains, of equal or even three times the size of human brains, was studied and tested. Finally, we investigated the usefulness of a panel of 20 antibodies (neuromarkers) associated with the normal structure and function of the brain, pathogens, age-related, and/or functional variations. The sampling protocol and some of the 20 neuromarkers have been thought to explore neurodegenerative diseases in these long-lived animals. To conclude, many of the typical measures used to evaluate neuropathological changes do not tell us if meaningful cellular changes have occurred. Having a wide panel of antibodies and histochemical techniques available allows for delving into the specific behavior of the neuronal population of the brain nuclei and to get a “fingerprint” of their real status.
ISSN: 2306-7381
DOI: 10.3390/vetsci9020038
Source: Veterinary Sciences [EISSN 2306-7381], v. 9 (2), 38, (Febrero 2022)
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