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Title: Caenorhabditis elegans to model the capacity of ascorbic acid to reduce acute nitrite toxicity under different feed conditions: Multivariate analytics on behavioral imaging
Authors: Verdu, Samuel
Perez, Alberto J.
Carrascosa Iruzubieta, Conrado Javier 
Barat, José M.
Talens, Pau
Grau, Raúl
UNESCO Clasification: 330990 Microbiología de alimentos
240117 Invertebrados
Keywords: Ascorbic acid
Behavior imaging
C. elegans
Multivariate analytics
Nitrite, et al
Issue Date: 2021
Project: University Polytechnic of Valencia by programme “Ayudas para la Contratación de Doctores para el Acceso al Sistema Español de Ciencia, Tecnología e Innovación, en Estructuras de Investigación de la UPV (PAID-10-17)”
Journal: International Journal of Environmental Research and Public Health 
Abstract: Nitrocompounds are present in the environment and human diet and form part of vegetables and processed meat products as additives. These compounds are related to negative impacts on human and animal health. The protective effect of ascorbic acid has been demonstrated by some biological systems as regards several nitrocompounds. This work focused on studying the possibility of modeling this effect on nitrite toxicity with the model Caenorhabditis elegans. The three factors studied in this work were ascorbic acid concentration, nitrite exposure concentration, and presence/absence of food. The protective effect was evaluated by scoring lethality and its impact on behavior by means of multivariate statistical methods and imaging analytics. The effects of nitrite and the influence of food availability were evidenced. Apart from increasing lethality, nitrite had disruption effects on movements. All the observed symptoms reduced when ascorbic acid was ad-ministered, and it diminished lethality in all cases. Ascorbic acid maintained nematodes’ postural capacities. The results suggest that nitrites’ nonspecific toxicity in C. elegans can be mitigated by ascorbic acid, as previously evidenced in other biological systems. Thus, our results reveal the ability of C. elegans to reproduce the known protective effect of ascorbic acid against nitrite.
ISSN: 1661-7827
DOI: 10.3390/ijerph18042068
Source: International Journal of Environmental Research and Public Health [ISSN 1661-7827], 18(4), 2068, (Febrero 2021)
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