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Title: Isolation and Characterization of a Bacillus velezensis D-18 Strain, as a Potential Probiotic in European Seabass Aquaculture
Authors: Monzón-Atienza, Luis 
Bravo, Jimena 
Torrecillas, Silvia 
Montero, Daniel 
Franco González-de Canales, Ana
García de la Banda, Inés
Galindo-Villegas, Jorge
Ramos-Vivas, José 
Acosta, Félix 
UNESCO Clasification: 251092 Acuicultura marina
Keywords: Bacillus velezensis D-18
Issue Date: 2021
Journal: Probiotics and Antimicrobial Proteins 
Abstract: Within the food-producing sectors, aquaculture is the one that has developed the greatest growth in recent decades, currently representing almost 50% of the world’s edible fish. The diseases can affect the final production in intensive aquaculture; in seabass, aquaculture vibriosis is one of the most important diseases producing huge economical losses in this industry. The usual methodology to solve the problems associated with the bacterial pathology has been the use of antibiotics, with known environmental consequences. This is why probiotic bacteria are proposed as an alternative fight against pathogenic bacteria. The aim of this study was to analyse a strain of Bacillus velezensis D-18 isolated from a wastewater sample collected from a fish farm, for use as probiotics in aquaculture. The strain was evaluated in vitro through various mechanisms of selection, obtaining as results for growth inhibition by co-culture a reduction of 30%; B. velezensis D-18 was able to survive at 1.5-h exposure to 10% seabass bile, and at pH 4, its survival is 5% and reducing by 60% the adhesion capacity of V. anguillarum 507 to the mucus of seabass and in vivo by performing a challenge. Therefore, in conclusion, we consider B. velezensis D-18 isolate from wastewater samples collected from the farms as a good candidate probiotic in the prevention of the infection by Vibrio anguillarum 507 in European seabass after in vitro and biosafety assays.
ISSN: 1867-1306
DOI: 10.1007/s12602-021-09782-8
Source: Probiotics and antimicrobial proteins [ISSN 1867-1306], v. 13, p. 1404–1412
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