Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/77186
Título: Erythropoietin treatment enhances muscle mitochondria capacity in humans
Autores/as: Plenge, Ulla
Belhage, Bo
Guadalupe-Grau, Amelia 
Andersen, Peter Riis
Lundby, Carsten
Dela, Flemming
Stride, Nis
Pott, Frank Christian
Helge, Jørn W.
Boushel, Robert
Clasificación UNESCO: 241106 Fisiología del ejercicio
Palabras clave: Humans
Mitochondria
Muscle
Oxidative Phosphorylation
RhEPO
Fecha de publicación: 2012
Publicación seriada: Frontiers in Physiology 
Resumen: Erythropoietin (Epo) treatment has been shown to induce mitochondrial biogenesis in cardiac muscle along with enhanced mitochondrial capacity in mice. We hypothesized that recombinant human Epo (rhEpo) treatment enhances skeletal muscle mitochondrial oxidative phosphorylation (OXPHOS) capacity in humans. In six healthy volunteers rhEpo was administered by sub-cutaneous injection over 8 weeks with oral iron (100 mg) supplementation taken daily. Mitochondrial OXPHOS was quantified by high-resolution respirometry in saponin-permeabilized muscle fibers obtained from biopsies of the vastus lateralis before and after rhEpo treatment. OXPHOS was determined with the mitochondrial complex I substrates malate, glutamate, pyruvate, and complex II substrate succinate in the presence of saturating ADP concentrations, while maximal electron transport capacity (ETS) was assessed by addition of an uncoupler. rhEpo treatment increased OXPHOS (from 92 ± 5 to 113±7pmol·s -1·mg -1) and ETS (107 ±4 to 143 ± 14pmol·s -1·mg -1, p< 0.05), demonstrating that Epo treatment induces an upregulation of OXPHOS and ETS in human skeletal muscle.
URI: http://hdl.handle.net/10553/77186
ISSN: 1664-042X
DOI: 10.3389/fphys.2012.00050
Fuente: Frontiers in Physiology [EISSN 1664-042X], v. 3, 50, (Marzo 2012)
Colección:Artículos
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