Isolation and characterization of microsatellite loci in Bencomia exstipulata and B. caudata (Rosaceae)

Abstract

Five novel polymorphic microsatellite markers were isolated from enriched libraries in order to examine population structure in the Canary Island endemic Bencomia exstipulata and the related Bencomia caudata. All markers were successfully amplified from both Bencomia species. They possessed an average of 6.8 and 7.0 alleles per locus in B. exstipulata and B. caudata, respectively. Our data are consistent with the tetraploid condition of both Bencomia species. In addition, this set of microsatellite loci offers an efficient tool to clarify the genetic relationships within the Bencomia alliance.