Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/47249
Título: Infectious pancreatic necrosis virus suppresses type I interferon signalling in rainbow trout gonad cell line but not in Atlantic salmon macrophages
Autores/as: Collet, Bertrand
Munro, Eann S.
Gahlawat, Suresh
Acosta, Felix 
Garcia, Jose 
Roemelt, Christina
Zou, Jun
Secombes, Christopher J.
Ellis, Anthony E.
Palabras clave: Double-Stranded-Rna
Mx Messenger-Rna
Antiviral Responses
Salar L.
Expression, et al.
Fecha de publicación: 2007
Editor/a: 1050-4648
Publicación seriada: Fish and Shellfish Immunology 
Resumen: RTG-P1 cells are a rainbow trout fibroblastic cell line permanently transfected with the luciferase gene under the control of the Mx promoter. On exposure to interferon (IFN) or IFN inducing agents, the cells produce luciferase. IPNV did not induce luciferase production up to 24 h post-infection but did not suppress constitutive luciferase production. Furthermore, IPNV suppressed luciferase production induced by poly I:C. RT-PCR analysis of IPNV infected cells showed IFN gene transcription from 6 h post-infection with increasing expression up to 24 h. Housekeeping genes beta-actin and GAPDH were also expressed along with upregulation of IRF1 and slight upregulation of STAT1. When RTG-P1 cells were stimulated with IFN, Mx transcripts, measured by qRT-PCR, peaked at 3-6 h and thereafter fell to low levels, but in the presence of IPNV, Mx transcription at this time was significantly suppressed but continued to rise gradually. Luciferase production was lower in infected cells at 12 h post-infection but not significantly after 24 h.These results indicate that, in non-stimulated RTG-P1 cells, while IPNV induces IFN transcription, activation of Mx expression is suppressed. Furthermore, when stimulated by IFN, the rate of Mx transcription is significantly suppressed by the virus. This would probably give time for the virus to replicate rapidly in the early phases of infection.Contrary to the fibroblastic cell line, IPNV stimulated IFN production by salmon macrophages in vitro at least as strongly as poly I:C, with no suppression of the IFN response to poly I:C, and the virus persisted for up to 9 days without causing CPE. (c) 2006 Published by Elsevier Ltd.
URI: http://hdl.handle.net/10553/47249
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2006.03.011
Fuente: Fish and Shellfish Immunology[ISSN 1050-4648],v. 22, p. 44-56
Colección:Artículos
Vista completa

Google ScholarTM

Verifica

Altmetric


Comparte



Exporta metadatos



Los elementos en ULPGC accedaCRIS están protegidos por derechos de autor con todos los derechos reservados, a menos que se indique lo contrario.