Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/44925
Título: Modulation of adrenocorticotrophin hormone (ACTH)-induced expression of stress-related genes by PUFA in inter-renal cells from European sea bass (Dicentrarchus labrax)
Autores/as: Montero, Daniel 
Terova, Genciana
Rimoldi, Simona
Tort, Lluis
Negrin, Davinia
Zamorano, María Jesús 
Izquierdo, Marisol 
Palabras clave: Polyunsaturated Fatty-Acids
Dietary Arachidonic-Acid
Regulatory Protein Expression
Seabream Sparus-Aurata
C-Fos, et al.
Fecha de publicación: 2015
Editor/a: 2048-6790
Proyectos: Advanced Research Initiatives For Nutrition & Aquaculture 
Publicación seriada: Journal of Nutritional Science 
Resumen: Dietary fatty acids have been shown to exert a clear effect on the stress response, modulating the release of cortisol. The role of fatty acids on the expression of steroidogenic genes has been described in mammals, but little is known in fish. The effect of different fatty acids on the release of cortisol and expression of stress-related genes of European sea bass (Dicentrarchus labrax) head kidney, induced by a pulse of adenocorticotrophin hormone (ACTH), was studied. Tissue was maintained in superfusion with 60 min of incubation with EPA, DHA, arachidonic acid (ARA), linoleic acid or a-linolenic acid (ALA) during 490 min. Cortisol was measured by RIA. The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real-time RT-PCR. There was an effect of the type of fatty acid on the ACTH-induced release of cortisol, values from ALA treatment being elevated within all of the experimental period. The expression of some steroidogenic genes, such as the steroidogenic acute regulatory protein (StAR) and c-fos, were affected by fatty acids, ALA increasing the expression of StAR after 1 h of ACTH stimulation whereas DHA, ARA and ALA increased the expression of c-fos after 20 min. ARA increased expression of the 11 beta-hydroxylase gene. Expression of heat shock protein 70 (HSP70) was increased in all the experimental treatments except for ARA. Results corroborate previous studies of the effect of different fatty acids on the release of cortisol in marine fish and demonstrate that those effects are mediated by alteration of the expression of steroidogenic genes.
URI: http://hdl.handle.net/10553/44925
ISSN: 2048-6790
DOI: 10.1017/jns.2015.6
Fuente: Journal of Nutritional Science [ISSN 2048-6790], v. 4 (e16)
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