Analysis of organochlorine pesticides in mollusc samples by HPLC after microwave assisted micellar extraction coupled with solid phase extraction

Abstract

A mixture of six organochlorine pesticides were determined by HPLC in different types of molluscs after their extraction by microwave assisted micellar extraction (MAME) coupled with a clean‐up and preconcentration step by solid phase extraction (SPE). A non‐ionic surfactant (Polyoxyethylene 10 lauryl ether, POLE) was used in MAME followed by SPE for cleaning and preconcentration of the samples. The extracts were determined by HPLC‐UV. With the optimum experimental variables established, the analytical parameters of the method were determined with satisfactory results (relative standard desviation values between 3.9 and 9.3% and detection limits between 6 and 38 ng · g−1). The method was validated using the conventional Soxhlet extraction method.