Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/128211
Título: Optimization and validation of a micro–QuEChERS method for phthalates detection in small samples of cetacean blubber
Autores/as: Sambolino, Annalisa
Rodriguez, Marta
De La Fuente Marquez,Jesus
Arbelo Hernández, Manuel Antonio
Fernandez Morales,Antonio
Kaufmann, Manfred
Cordeiro, Nereida
Dinis, Ana
Clasificación UNESCO: 3109 Ciencias veterinarias
Palabras clave: Bottlenose Dolphin
Gas Chromatography-Mass Spectrometry
Gc–Ms
Phthalic Acid Esters
Pilot Whale, et al.
Fecha de publicación: 2024
Publicación seriada: MethodsX 
Resumen: In this study, an innovative method was developed to detect and quantify phthalates in fresh cetacean blubber. An adaptation of the ammonium formate QuEChERS method was used and adapted as a micro-extraction for small quantities of samples. Significantly, this technique utilized minimal quantities of reagents and salts, with the additional implementation of rigorous Quality Assurance/Quality Control protocols to further reduce background contamination. To ensure the reliability of this method, comprehensive validation procedures were conducted, with a specific focus on two widely studied cetacean species: the common bottlenose dolphin (Tursiops truncatus) and the short-finned pilot whale (Globicephala macrorhynchus). Determination coefficients (R2) for matrix-matched calibration were >0.93 with limits of quantifications (LOQ) of the method in the range of 5–10 ng/g. Mean recovery values were between 40 and 100 %. This novel methodology holds particular relevance for environmental research studies, offering the capability to detect emerging contaminants with minimal sample requirements. This aspect is particularly valuable in investigations that involve free-ranging animals and rely on biopsy sampling. It allows for the assessment of contaminant levels in healthy individuals within wild populations, enhancing our understanding of ecological impacts and potential conservation measures. • A micro-extraction adaptation of the ammonium formate QuEChERS method was developed and applied to a small quantity of fresh cetacean blubber to detect phthalates. • Small quantities of reagents and salts were used, and additional Quality Assurance/ Quality Control procedures were taken to further minimize background contamination. • Method validation was carried out for two cosmopolitan and extensively studied cetacean species: the common bottlenose dolphin (Tursiops truncatus) and the short-finned pilot whale (Globicephala macrorhynchus).
URI: http://hdl.handle.net/10553/128211
DOI: 10.1016/j.mex.2023.102502
Fuente: MethodsX[EISSN 2215-0161],v. 12, (Junio 2024)
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