Empleo de diferentes pruebas serológicas para Trypanosoma evansi en cabras inoculadas experimentalmente

Abstract

Animals suffering from Trypanosoma evansi infection in the Canary Islands have been treated but dissemination of the disease in several hosts is not excluded. Particularly goats could play an important role in the epidemiology of T. evansi in the Canary Islands. Thus, the objective of this work was to assess the performance of simple and rapid serological antibody tests in experimentally inoculated goats. Five adult goats were inoculated intravenously with, at least, 1 x 105 T. evansi, they were kept for 8 months and monthly checked for the presence of the parasite and of specific antibodies. The serological tests investigated were the direct card agglutination test CATT/T. evansi and the indirect card agglutination test LATEX/T. evansi. The inoculated goats showed a subclinical course of the disease. Parasitemia remained very low but was persistent. All animals became positive in the CATT/T. evansi after one month PI and remained positive with minimum end-titer of 1/4. Similar results were obtained with the LATEX/T.evansi although at lower endtiters (1/2). We conclude that CATT/T. evansi is adequate for assessing infection of Canarian goats by T. evansi.