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http://hdl.handle.net/10553/107537
Título: | Seminal plasma Alters surface Glycoprofile of dromedary camel cryopreserved epididymal spermatozoa | Autores/as: | Desantis, Salvatore Lacalandra, Giovanni Michele Batista Arteaga, Miguel Amann, Olga Antonelli, Dario Monaco, Davide |
Clasificación UNESCO: | 310411 Reproducción | Palabras clave: | Lectin-Binding Sites In-Vitro Acrosome Reaction Cytoplasmic Droplets Fertilizing Ability, et al. |
Fecha de publicación: | 2021 | Publicación seriada: | Theriogenology | Resumen: | The high viscosity of Camelidae semen continues to present a major impediment for its application in assisted reproduction technology. The exposure of epididymal spermatozoa (ES) to seminal plasma (SP) may provide an approach to enhance the development of assisted reproductive techniques in these important domestic species. Since the sperm glycocalyx plays a key role in reproduction we aimed to evaluate whether SP exposure modifies the surface glycosylation patterns of cryopreserved dromedary ES. Epididymal sperm was collected through retrograde flushing of the cauda epididymidis that were obtained from orchidectomized mature dromedary bulls. The collected samples were then cryopreserved after dilution with a tris citrate clarified egg yolk extender, with and without the supplementation of 15% SP. Post-thaw carbohydrate surface profiles of both control and SP-treated spermatozoa were analyzed using 15 fluorescent lectins. Morpho-functional properties were also investigated via computer assisted sperm analysis. Lectin-binding analysis of the glycocalyx in control sperm revealed the presence of (1) N-glycans terminating with lactosamine (Con A, PHA-L, and RCA(120)), in both acrosomal and tail regions. Whilst (2) alpha 2,3-/alpha 2,6-linked sialic acids (MALII, SNA), and O-linked glycans terminating with a single N-acetylgalactosamine residue (Tn antigen) (HPA, SBA) along with galactoseb1,3N-acetylgalactosamine (T antigen) (PNA) were observed in the acrosomal cap. The expression of both N-acetylglucosamine (sWGA and GSA II) and terminalagalactose (GSA I-B-4) residues was also noted in the acrosomal cap region of control sperm. Compared with controls, SP treated samples displayed: 1) the appearance of bisected ditriantennary complex-type N-glycans (PHA-E), terminating with lactosamine, as well as an increase of Oglycans terminating with Tn and T antigens in both the acrosomal and tail regions; 2) an increase in glycans containing alpha 2,6-linked sialic acid, N-acetylglucosamine, and agalactose in the tail region. The cytoplasmic droplets of both control and seminal plasma-treated sperm bound Con A, PHA-E, PHA-L, RCA(120), HPA, PNA, sWGA, GSA I-B-4, and GSA II. These results indicate that SP treatment affects the glycan composition of the dromedary camel ES glycocalyx. More comprehensive studies are required in order to evaluate the fertilization capacity of SP-treated ES in order to facilitate its application in dromedary camel assisted reproduction technology. | URI: | http://hdl.handle.net/10553/107537 | ISSN: | 0093-691X | DOI: | 10.1016/j.theriogenology.2021.03.008 | Fuente: | Theriogenology [ISSN 0093-691X], v. 167, p. 77-84, (Junio 2021) |
Colección: | Artículos |
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