Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/848
Title: Morphogenetic effect of glycerol on tissue cultures of the red seaweed Grateloupia doryphora
Authors: Robaina Romero, Rafael 
Garcia, P. 
Luque-Escalona, Angel 
García-Jiménez, Pilar 
Keywords: Algas rojas
Glicerina
Carbon-source
Glycerol
Grateloupia doryphora
Morphogenesis
Osmolality
Solidity
Tissue culture
Issue Date: 1990
Journal: Journal of Applied Phycology 
Abstract: Explants of Grateloupia doryphora were cultivated in Provasoli Enriched Seawater culture medium (PES) supplemented with glycerol (0.1, 0.3, 0.5 or 0.8 mol 1−1) or carbohydrates (0.1 or 0.3 mol 1−1 mannose, glucose and galactose) and agar (3, 8, 15 g 1−1 ). The osmolality of the medium was adjusted by dilution of the seawater (70 or 100%, v/v). The increase in fresh weight of explants cultivated in liquid medium with glycerol (0.3 mol 1−1) and without glycerol was compared. All experiments were carried out in the light, except for one assay in which the explants were cultivated in the dark. Glycerol was an effective carbon source for the vegetative propagation of G. doryphora in solid and liquid media. Mannose, glucose and galactose all had no effect on growth or morphogenesis of the explants. In solid media the main effect of glycerol was as a morphogenetic inductor, with PES70 (70% seawater) + 0.1 or 0.3 mol 1−1 glycerol + 3 or 8 g 1−1 agar the best formulation. An increase in the concentration of agar in glycerol-containing medium reduced the morphogenetic capacity of the explants, which developed into compact cell masses. The effects of glycerol were observed only in explants cultivated under light.
URI: http://hdl.handle.net/10553/848
ISSN: 0921-8971
DOI: 10.1007/BF00023375
Source: Journal of Applied Phycology [ISSN 0921-8971], v. 2 (2), p. 137-143
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