Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/77062
Title: Development of whole-genome multiplex assays and construction of an integrated genetic map using SSR markers in Senegalese sole
Authors: Guerrero-Cózar, Israel
Pérez García, Cathaysa 
Benzekri, Hicham
Sánchez Medina, Javier Jesús
Seoane, Pedro
Cruz, Fernando
Gut, Marta
Zamorano Serrano, María Jesús 
Claros, M. Gonzalo
Manchado, Manuel
UNESCO Clasification: 240108 Genética animal
251092 Acuicultura marina
Keywords: Solea senegalensis
Flatfishes
Fish genetics
Microsatellites (Genetics)
Nucleotide sequence, et al
Issue Date: 2020
Journal: Scientific Reports
Abstract: The Senegalese sole (Solea senegalensis) is an economically important flatfish species. In this study, a genome draft was analyzed to identify microsatellite (SSR) markers for whole-genome genotyping. A subset of 224 contigs containing SSRs were preselected and validated by using a de novo female hybrid assembly. Overall, the SSR density in the genome was 886.7 markers per megabase of genomic sequences and the dinucleotide motif was the most abundant (52.4%). In silico comparison identified a set of 108 SSRs (with di-, tetra- or pentanucleotide motifs) widely distributed in the genome and suitable for primer design. A total of 106 markers were structured in thirteen multiplex PCR assays (with up to 10-plex) and the amplification conditions were optimized with a high-quality score. Main genetic diversity statistics and genotyping reliability were assessed. A subset of 40 high polymorphic markers were selected to optimize four supermultiplex PCRs (with up to 11-plex) for pedigree analysis. Theoretical exclusion probabilities and real parentage allocation tests using parent-offspring information confirmed their robustness and effectiveness for parental assignment. These new SSR markers were combined with previously published SSRs (in total 229 makers) to construct a new and improved integrated genetic map containing 21 linkage groups that matched with the expected number of chromosomes. Synteny analysis with respect to C. semilaevis provided new clues on chromosome evolution in flatfish and the formation of metacentric and submetacentric chromosomes in Senegalese sole.
URI: http://hdl.handle.net/10553/77062
DOI: 10.1038/s41598-020-78397-w
Source: Scientific reports [EISSN 2045-2322], v. 10 (1), (Diciembre 2020)
Appears in Collections:Artículos
Thumbnail
PDF
Adobe PDF (3,92 MB)
Show full item record

SCOPUSTM   
Citations

11
checked on Jun 16, 2024

WEB OF SCIENCETM
Citations

10
checked on Jun 16, 2024

Page view(s)

126
checked on May 11, 2024

Download(s)

93
checked on May 11, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.