Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/76978
Title: Is sperm cryopreservation at-150 degrees C a feasible alternative?
Authors: Medrano, A.
Cabrera Martín, Fernando 
González Valle, Fernardo
Batista Arteaga, Miguel 
Gracia Molina, Anselmo 
UNESCO Clasification: 310411 Reproducción
Keywords: Spermatozoa
Semen
Goat
Freezing
Ultra-Low Temperature Freezer
Issue Date: 2002
Journal: Cryo-Letters 
Abstract: A series of experiments was carried out to validate a -150degreesC ultra-low temperature freezer for its possible use to properly freeze and store semen. In the first part, crude sample handling was simulated to see whether temperature of stored samples was maintained within a safe range; also, the freezing point and latent heat of fusion plateau of a semen extender were monitored. In the second part, buck semen was (i) frozen in liquid nitrogen and stored in the ultra-low freezer, (ii) frozen and stored in the ultra-low freezer, and (iii) frozen and stored in liquid nitrogen, to compare sperm cryosurvival between freezing methods. Both, frequent removal of samples and long opening of the freezer door did not negatively affect stored sample temperature; latent heat of fusion plateau was 5 minutes long. Semen stored either at -150degreesC or at -196degreesC cryosurvived similarly after 2 days and after 2 months of cryopreservation.
URI: http://hdl.handle.net/10553/76978
ISSN: 0143-2044
Source: Cryoletters [ISSN 0143-2044], v. 23 (3), p. 167-172, (Mayo-Junio 2002)
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