Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/76972
Title: Morphological and functional characterization of beige mouse adrenomedullary secretory vesicles
Authors: Borges, Ricardo
Jaén, Ruth
Freire, Francisco
Gómez, José F.
Villafruela, Cristina
Yanes, Eulalia
UNESCO Clasification: 2407 Biología celular
Keywords: Amperometry
Exocytosis
Mast Cells
Chediak-Higashi Syndrome
Microelectrodes, et al
Issue Date: 2001
Journal: Cell and Tissue Research 
Abstract: We tested whether the giant secretory granules observed in the mast cells of the naturally occurring mutant beige mouse (BM) (C57BL/6N-bg) were also present in the adrenal chromaffin cells. The presence of large chromaffin granules (CG) would be a valuable tool for the study of exocytosis in neuronal tissues. Conversely, the observation of large vesicles within chromaffin cells that are different from CG could indicate that CC are of a different origin than granules of mast cells. Ultrastructural analysis demonstrated the presence of large lysososmal-like vesicles in the BM, and also a discrete increase in the number of CG with diameters larger than 240 nm but not of giant CC. In addition, amperometric measurements of single-event exocytosis, using carbon fiber microelectrodes, showed no differences between the quantal size of secretory events from BM and wildtype or bovine chromaffin cells. Minor but significant differences were found between the kinetics of exocytosis in SM cells and wild-type mouse cells. We conclude that CG, but not the abnormal-sized vesicles found in BM chromaffin cells contribute to the catecholamine secretion and that abnormal secretory granules are not present in adrenergic cell lineage.
URI: http://hdl.handle.net/10553/76972
ISSN: 0302-766X
DOI: 10.1007/s004410000320
Source: Cell and Tissue Research [ISSN 0302-766X], v. 304 (1), p. 159-164, (Abril 2001)
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