Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/76910
Title: Effect of cyclosporin A on inflammatory cytokine production by U937 monocyte-like cells
Authors: Losa García, Juan E.
Martín de Cabo, María Rosa
Mateos Rodríguez, Fernando
Pérez Losada, Jesús
Jiménez López, Antonio
Pérez Arellano, José Luis 
UNESCO Clasification: 320505 Enfermedades infecciosas
Keywords: Cyclosporin
Cytokine
U937
Macrophage
Monocyte
Issue Date: 2000
Journal: Mediators of Inflammation 
Abstract: CYCLOSPORIN A (CsA) is an immunosuppresor drug that has been used in the treatment of several types of inflammatory diseases. In some of them the inhibition of T-lymphocyte activation does not suitably account for the observed beneficial effect, suggesting that CsA could act on other types of cells. The present study was undertaken to determine the effect of CsA on inflammatory cytokine secretion by U937 monocyte cells. Undifferentiated and dimethylsulfoxide ;(DMSO) differentiated U937 cells were incubated with different concentrations of CsA (200, 20 and 2 ng/mL) in the presence or absence of phorbol-myristate acetate (PMA). Interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8 levels were measured in supernatants using specific enzyme-linked immunosorbent assays. At the highest concentration used (200 ng/mL) CsA decreased the basal and stimulated secretion of all the inflammatory cytokines studied in both undifferentiated and differentiated cells, with the only exception of PMA-stimulated IL-1 secretion by undifferentiated cells. However, only basal secretion of interleukin-8 in both undifferentiated and DMSO-differentiated U937 cells was significantly reduced by CsA at the highest concentration (200 ng/ mL). At therapeutic concentrations in vivo, CsA exerts a predominant effect on IL-8 secretion by human mononuclear phagocytes.
URI: http://hdl.handle.net/10553/76910
ISSN: 0962-9351
DOI: 10.1080/09629350020008682
Source: Mediators of Inflammation [ISSN 0962-9351], v. 9 (3-4), p. 169-174, (2000)
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