Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/75549
Title: Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp.
Authors: Gongi, Wejdene
Cordeiro, Nereida
Pinchetti, Juan Luis Gómez 
Sadok, Saloua
Ben Ouada, Hatem
UNESCO Clasification: 241707 Algología (ficología)
Keywords: Antioxidant Enzymes
Characterization
Chlorophyta
Epss
Graesiella
Microalgae
Outdoor Cultivation
Radical Scavenging
Two-Step Strategy
Issue Date: 2020
Project: Red de Excelencia en Biotecnología Azul (Algas) de la Región Macaronésica. Consolidación, Certificación y Transferencia 
Journal: Journal of Applied Phycology 
Abstract: The present study developed a two-step strategy to enhance the production of extracellular polymeric substances (EPSs) by a thermotolerant chlorophyte, Graesiella sp., in view to their industrial valorisation. In the first step, Graesiella sp. was grown in outdoor conditions in pilot-scale photobioreactors of 100 L culture volumes. In the second step, the biomass collected in the exponential growth phase was submitted to heat stress (50 °C). A joint production of biomass reaching 0.50 gdw L−1 day−1 and of EPS production reaching 1.30 gdw L−1 in 2 days was obtained. EPSs mainly contained polysaccharides (80%) and proteins (14%). FTIR and 1HNMR revealed the presence of primary amine and sulfated groups. The EPSs contained antioxidant enzymes (SOD, CAT, and APX) maintained in an active state by the microenvironment offered by the EPSs. The EPSs were found to have a potent antioxidant activity via directly scavenging free radicals when compared to l-ascorbic acid.
URI: http://hdl.handle.net/10553/75549
ISSN: 0921-8971
DOI: 10.1007/s10811-020-02303-0
Source: Journal of Applied Phycology [ISSN 0921-8971], (Noviembre 2020)
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