Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/75257
Title: Proteomic profile and proteases characterisation of greater amberjack skin mucus after Neobenedenia girellae infection
Authors: Fernández Montero, Álvaro 
Torrecillas Burriel,Silvia 
Acosta, F. 
Prieto-Alamo, M. J.
Jurado, J.
Montero, D. 
UNESCO Clasification: 251092 Acuicultura marina
Keywords: Greater Amberjack
Mucus
Skin
Proteomic
Ectoparasites
Issue Date: 2019
Publisher: Elsevier 
Journal: Fish and Shellfish Immunology 
Conference: 3rd Conference of the International Society of Fish & Shellfish Immunology (ISFSI 2019) 
Abstract: Skin mucus is known for being the first physical and immunological barrier in fish. Skin mucus is composed by a wide range of proteins, like glycoproteins, structural proteins, metabolic proteins and immune related protein-components. Likewise, proteome changes in Atlantic salmon (Salmo salar) have been observed due to sea lice infections, demonstrating the importance of protein-immune defenses against ectoparasite infections. Nowadays, greater amberjack (Seriola dumerili) pass-through a biosanitary bottleneck on its on-growing period related with monogenean ectoparasites, which could cause a 90% of mortality (Ogawa et al.,1998). For that reason, this study aimed to compare skin mucus proteome of non-infected and experimentally infected greater amberjack juveniles with Neobenedenia girellae, as well as to characterize proteases of this skin mucus. Thirty greater amberjack juveniles of 150 ±12 g were randomly distributed in 3 cylindroconical tanks of 500 liters. After 10 days of acclimation, skin mucus of non-infected fish was obtained, pooled and immediately freeze in liquid nitrogen. Cohabitation with N.girellae was conducted with 3 previously infected fish stored in cages for 15 days, when all the experimental fish were infected, skin mucus was sampled. The integrative proteomic approach was conducted using a label-free procedure as LC-MS/MS with a 2-DE-PMF-MS/MS. Protease activity was conducted using azocasein hydrolysis assay, while protease characterization was determined combining azocasein hydrolysis with inhibitors of metalloproteases and serine proteases. Results obtained with LC-MS/MS showed the first microbiota analyses in greater amberjack skin mucus, were the most abundant species belonged to gamma-proteobacteria group, and infected and non-infected fish bacterial presence only differed in 6 genus of bacteria.2-DE-PMF-MS/MS analyses showed differences in proteome profile at a qualitative level. Proteins of p/ 5 and molecular weight ranging between 36-66 KD, typically identified as structure proteins, were clearly affected by degradation for N.girellae infected fish. Protease activity analysis showed no difference among infected and non-infected fish, however proteases populations differed in metalloproteases and serine proteases when comparing infected and noninfected fish.
URI: http://hdl.handle.net/10553/75257
ISSN: 1050-4648
DOI: 10.1016/j.fsi.2019.04.268
Source: Fish and Shellfish Immunology [ISSN 1050-4648], v . 91, p. 463-464, (Agosto 2019)
Appears in Collections:Actas de congresos
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